YMC CHIRAL PREP CD ST/PM 手性分離管柱(不對稱/鏡相)
YMC CHIRAL PREP CD ST/PM are suitable packing materials for preparative isolation of optical isomers. ST is suitable for use mainly in reversed-phase mode, while PM can be used in both normal-phase and reversed-phase modes. The cost is considerably less than that of the conventional packing materials for optical isomers, and therefore YMC CHIRAL PREP CD ST/PM are appropriate not only for semi-preparative isolation but also for industrial isolation. They are also applicable to simulated moving bed(SMB) chromatography and axial compression columns.
SPECIFICATION
| Particle size | : 10µm, 20µm, 50µm |
|---|---|
| Pore size | : 120Å |
| Usable pH range | : 2.0-7.0 |
POINT
- β-cyclodextrin modified silica gel packing material
- Can separate various optical isomers
- Can be used in both normal-phase and reversed-phase modes
- Applicable to large-scale isolation by simulated moving bed(SMB) chromatography
- Various particle sizes are available
- Bulk supply at kilogram scale is available
- Excellent cost performance
Features
As the differences in the hydrophobicities of the cis-trans isomers of piperine, which is a pungent component of pepper, are small, commonly used reversed phase columns are not suitable for their separation. However, YMC-Triart PFP provides excellent separations as it recognizes minor charge localizations in a molecule owing to various binding through as π–π and dipole–dipole interactions. YMC-Triart PFP shows high selectivity towards compounds with small structural differences.
Colomn:YMC CHIRAL PREP CD ST(10µm, 120Å)
Scale up from analytical to preparative conditions is fast and easy with high sample loading.
| Optical isomer | Column | Load* | Optical purity(F1) | Recovery amount(F1) | Recovery rate(F1) |
|---|---|---|---|---|---|
| 1. | PM | 100mg | >99.9% | 49.6mg | 99.2% |
| 2. | ST | 100mg | >99.9% | 47.7mg | 94.8% |
| 3. | ST | 100mg | >99.9% | 38.5mg | 77.0% |
| 4. | PM | 200mg | >99.9% | 96.5mg | 96.5% |
| 5. | PM | 250mg | >99.9% | 114.2mg | 91.3% |
*Column size :250X20mm I.D. (10µm, 120Å)
Highly pure optical isomers can be obtained with high recovery.
| Column | : YMC CHIRAL PREP CD PM (10µm,120Å) 250X4.6 mm I.D. |
|---|---|
| Eluent | : hexane /ethanol (95/5) |
| Flow rate | : 0.5mL/min |
| Temperature | : ambient |
| Detection | : UV at 254nm |
| Injection | : 5µL (1mg/mL) |
| Column | : YMC CHIRAL PREP CD ST (0µm,120Å) 250X4.6 mm I.D. |
|---|---|
| Eluent | : 20mM KH2PO4 (pH4.6) /acetonitrile (99/1) |
| Flow rate | : 1.0mL/min |
| Temperature | : ambient |
| Detection | : UV at 220nm |
| Injection | : 5µL (1mg/mL) |
Besides water, alcohol or acetonitrile, hexane or tetrahydrofuran can be used as mobile phase.
