Alcyon SFC 超臨界流體層析管柱
Alcyon SFC columns are Supercritical Fluid Chromatography (SFC) columns available with chiral/achiral stationary phases. Alcyon SFC columns are specifically packed in SFC-compatible hardware and are tested under SFC conditions.
The low viscosity of supercritical carbon dioxide allows for analytical separations that are 3–5 times faster than those for normal-phase HPLC.
POINTS
￭ Available in chiral and achiral stationary phases ￭ Faster separation with high resolution ￭ Excellent durability ￭ Great reduction of solvent consumption
Features
[ Chiral Immobilized type ]
|
Particle size
(µm) |
Chiral selector
|
Column size*
inner diameter X length (mm) |
||
|---|---|---|---|---|
| Alcyon SFC CSP Amylose-SA | 3 5 |
 |
 |
2.1 X 150 3.0 X 50 3.0 X 100 3.0 X 150 4.6 X 150 4.6 X 250 10 X 250 20 X 250 |
| Alcyon SFC CSP Cellulose-SB |  |
|||
| Alcyon SFC CSP Cellulose-SC |  |
 |
||
 Alcyon SFC CSP Cellulose-SJ |
 |
 |
||
[ Chiral Coated type ]
|
Particle size
(µm) |
Chiral selector
|
Column size*
inner diameter X length (mm) |
||
|---|---|---|---|---|
| Alcyon SFC CSP Amylose-C Alcyon SFC CSP Amylose-C Neo |
3 5 |
|
|
2.1 X 150
3.0 X 50 3.0 X 100 3.0 X 150 4.6 X 150 4.6 X 250 10 X 250 20 X 250 |
| Alcyon SFC CSP Cellulose-C | |
|||
[ Achiral ]
|
Particle size
(µm) |
Functional group
|
Column size
inner diameter X length (mm) |
|
|---|---|---|---|
| Alcyon SFC Triart C18 |
5
|
-C18H37
|
2.1 X 150
4.6 X 150 4.6 X 250 |
| Alcyon SFC Triart Diol |
 |
||
| Alcyon SFC Triart PFP |
 |
||
| Alcyon SFC CN |
-CH2CH2CH2CN
|
2.1 X 150
4.6 X 150 4.6 X 250 10 X 250 20 X 250 |
|
| Alcyon SFC SIL |
(-OH)
|
Faster chiral separation of trans-Stilbene oxide is achieved using supercritical fluid chromatography compared with HPLC separation. The lower viscosity and larger diffusion coefficient of the supercritical fluid provide rapid separation of both chiral and achiral compounds.
[ SFC condition ]
| Column | ： | Alcyon SFC CSP Amylose-C 5 µm, 250 X 4.6 mmI.D. |
|---|---|---|
| Eluent | ： | CO2/methanol (60/40) |
| Flow rate | ： | 3.0 mL/min |
| Temperature | ： | 40ºC |
| Detection | ： | UV at 220 nm |
| Back pressure | ： | 17.2 MPa (2500 psi) |
[ HPLC condition ]
| Column | ： | CHIRAL ART Amylose-C 5 µm, 250 X 4.6 mmI.D. |
|---|---|---|
| Eluent | ： | n-hexane/2-propanol (90/10) |
| Flow rate | ： | 1.0 mL/min |
| Temperature | ： | 25ºC |
| Detection | ： | UV at 220 nm |
Excellent peak shape of 2-Phenylpropionic acid is obtained using SFC chiral separation. Under HPLC conditions, the peak shape is very broad with mobile phases containing no additives, such as acid. However, using SFC, the peak shapes are very good with just a mixture of CO2 and methanol. Supercritical carbon dioxide is considered to act as an weak acid.
[ SFC condition ]
| Column | ： | Alcyon SFC CSP Cellulose-C 5 µm, 250 X 4.6 mmI.D. |
|---|---|---|
| Eluent | ： | CO2/methanol (98/2) |
| Flow rate | ： | 3.0 mL/min |
| Temperature | ： | 35ºC |
| Detection | ： | UV at 220 nm |
| Back pressure | ： | 13.8 MPa (2000 psi) |
[ HPLC condition ]
| Column | ： | CHIRAL ART Cellulose-C 5 µm, 250 X 4.6 mmI.D. |
|---|---|---|
| Eluent | ： | n-hexane/2-propanol (99/1) |
| Flow rate | ： | 1.0 mL/min |
| Detection | ： | UV at 220 nm |
The results of analyzing phenols on three kinds of achiral columns are shown. As the separation behavior is different, a column can be selected to suit the sample characteristics.
YMC offers five achiral-phase SFC columns.
[ Alcyon SFC Triart Diol ]
[ Alcyon SFC SIL ]
[ Alcyon SFC CN ]
| Column | ： | 5 µm, 250 X 4.6 mmI.D. |
|---|---|---|
| Eluent | ： | CO2/methanol (88/12) |
| Flow rate | ： | 3.0 mL/min |
| Temperature | ： | 30ºC |
| Detection | ： | UV at 230 nm |
| Back pressure | ： | 10.3 MPa (2000 psi) |
Alcyon SFC columns show excellent peak shape, even for faster separations. As a result, high-purity and high-recovery purification will be achieved. Alcyon SFC columns offer purification with higher efficiency owing to lower solvent consumption.
| SFC | Fr.1 | Fr.2 |
|---|---|---|
| Enantiomeric purity | >99.9 %ee | 99.8 %ee |
| Yield | 94.5 % | 95.6 % |
| Productivity* | 340 (mg product/hr) |
344 (mg product/hr) |
| Column | ： | Alcyon SFC CSP Amylose-C 5 µm, 250 X 20 mmI.D. |
|---|---|---|
| Eluent | ： | CO2/ethanol (80/20) |
| Flow rate | ： | 60 mL/min |
| Temperature | ： | 30ºC |
| Detection | ： | UV at 280 nm |
| Back pressure | ： | 15 MPa (2180 psi) |
| Injection | ： | 1.5 mL (20 mg/mL) |
*Calculated based on injections of every 2.5 minutes on SFC.
« High column durability under repeated backpressure gradient conditions »
The results of sequential analysis under backpressure gradient conditions on Alcyon SFC CSP Amylose-C are shown.
The theoretical plate number and retention time are maintained, even after the sequential gradient test. The Alcyon SFC column has excellent durability under such tough conditions.
Change rate of theoretical plate number
Change rate of retention time
Durability test
| Column | ： | Alcyon SFC CSP Amylose-C 5 µm, 250 x 4.6 mmI.D. |
|---|---|---|
| Eluent | ： | CO2/methanol (80/20) |
| Flow rate | ： | 1.0 mL/min |
| Temperature | ： | 50ºC |
| Back pressure | ： | 10.3 -24.1 MPa (1500-3500 psi) (0-10 min) 10.3 MPa (1500 psi) (10-13 min) |
Column performance test (every 20 gradient cycles)
| Column | ： | Alcyon SFC CSP Amylose-C 5 µm, 250 x 4.6 mmI.D. |
|---|---|---|
| Eluent | ： | CO2/methanol (80/20) |
| Flow rate | ： | 3.0 mL/min |
| Temperature | ： | 50ºC |
| Detection | ： | UV at 220 nm |
| Back pressure | ： | 10.3 MPa (1500 psi) |
| Sample | ： | trans-Stilbene oxide |
