YMC-Triart Bio C18 層析管柱
YMC-Triart Bio C18採用有機雜化矽膠(hybrid silica-based)的寬孔徑(wide-pore)C18液相層析管柱，適用於分離肽，蛋白質和寡核苷酸等較大分子量之生物樣品。
另外，由於其低疏水性(low hydrophobicity)，相較於一般常見之C18層析管柱，滯留時間較短，可應用於快速的樣品分析
| SPECIFICATION | |
| Base | organic/inorganic hybrid silica |
|---|---|
| Particle size | 1.9 µm, 3 µm, 5 µm |
| Pore size | 300 Å |
| Usable pH range | 1-12 |
| USP | L1 |
Features
The effects of pore size on separation of peptides and proteins
The widths at half the peak height (WHPH) were compared when peptides and proteins with molecular weight (MW) from 1,859 to 76,000 were separated using Triart Bio C18 and Triart Bio C4 with pore size 300 Å, and Triat C18 with pore size 120 Å. Good peak shape was obtained using Triart Bio C18 and Triart Bio C4, which are wide-pore columns, even in separation of proteins with high MW. On the other hand, peak broadening was observed when using Triart C18 (120 Å) on separation of proteins with MW over 10,000. Wide-pore columns with pore size 300 Å are suitable for separation of proteins with MW over 10,000.
| Column | 150 X 3.0 mmI.D. |
|---|---|
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 10-95%B (0-15 min) |
| Flow rate | 0.425 mL/min |
| Temperature | 40ºC |
| Detection | UV at 220 nm |
| Injection | 4 µL (0.1-0.5 mg/mL) |
| Sample | γ-Endorphin (MW 1,859), Insulin (MW 5,733), Lysozyme (MW 14,000), β-Lactoglobulin (MW 18,363), α-Chymotrypsinogen A (MW 25,656), BSA (MW 66,000), Conalbumin (MW 76,000) |
Separation example of Human growth hormone (MW 22,000)
This example shows that chromatograms of Human growth hormone, a peptide with MW 22,000, which was separated using Triart Bio C18 and Triart Bio C4 with pore size 300 Å, and Triart C18 with pore size 120 Å, respectively. Good peak shape can be obtained on analysis of high MW molecules such as proteins with Triart Bio C18 and Triart Bio C4, wide-pore columns. Excellent separation was achieved using Triart Bio C18 with longer alkyl chain in its bonded phase.
| Column | 150 X 3.0 mmI.D. |
|---|---|
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.08) 50-70%B (0-15 min) |
| Flow rate | 0.425 mL/min |
| Temperature | 40ºC |
| Detection | UV at 220 nm |
| Injection | 8 µL |
| Sample | Human growth hormone (0.1 mg/mL) |
Triat Bio C18 is suitable for highly sensitive analysis and structural analysis of proteins using LC-MS since it gives good peak shape in mobile phase containing formic acid.
| Column | 150 X 3.0 mmI.D., 150 X 4.6 mmI.D. |
|---|---|
| Eluent | A) water/formic acid (100/0.1) B) acetonitrile/formic acid (100/0.08) 45-65%B (0-15 min) |
| Flow rate | 0.425 mL/min for 3.0 mmI.D. 1.0 mL/min for 4.6 mmI.D. |
| Temperature | 40ºC |
| Detection | UV at 220 nm |
| Sample | Human growth hormone (0.1 mg/mL) |
