YMC-Pack Ph 層析管柱
Unique Selectivity
Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed phases such as ODS, C8 and C4. The π electrons of the phenyl group can interact with the aromatic ring of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to ODS and C4, for the analysis of peptides and proteins, on both small pore 120Å and wide-pore 300Å silica supports. Retention is decreased on wide-pore phenyl phases relative to 120Å phenyl material. Generally, as pore size increases surface area decreases, resulting in shorter retention times.
SPECIFICATION
| Particle size | : 3µm, 5µm 10µm |
|---|---|
| Pore size | : 120Å |
| Carbon contents | : 9% |
| Usable pH range | : 2.0-7.5 |
POINT
￭ Monomeric phenyl, fully endcapped
￭ Preferential retention of aromatic compounds
￭ Alternative to ODS or C4 bonded phases for the analysis or peptides and other biomolecules
Features
This figure shows difference in retention behavior of Ph and ODS for separation of water-soluble vitamins.
- p-Aminobenzoic acid (PABA)
- Biotin (Vitamin H)
- Riboflavin (Vitamin B2)
- Cyanocobalamin (Vitamin B12)
