YMC-Pack PROTEIN-RP 層析管柱

YMC-Pack PROTEIN-RP is a reversed-phase column utilizing a silica gel base. It contains a stationary phase, specifically designed for separation of proteins or peptides.
Problems that are associated with conventional reversed-phase columns with short alkyl chain lengths are minimized.Robust column lifetime and excellent recovery of hydrophobic proteins are typically possible on this phase.

SPECIFICATION

Particle size	: 5µm
Pore size	: 200Å
Usable pH range	: 1.5-7.5

POINT

-Improved recovery of proteins or peptides
-Improved durability when used with TFA solution
-Enabled elution of high molecular weight proteins

Features

Improved recovery of proteins or peptides

Sample	Recovery (%)
Sample	YMC-Pack PROTEIN-RP	C4 column, Company A	C4 column, Company B
Ribonuclease A	93	95	86
Cytochrome c	94	89	95
Lysozyme	98	93	76
Myoglobin	97	85	93
BSA	94	92	86
Ovalbumin	90	73	88
Transferrin	94	98	88
Insulin(bovine)	97	73	92
Insulin B chain	82	76	70
α-Mating factor	93	82	81
Leu-Enkephalin	92	84	91
Gly-Gly-Gly-Gly	95	86	70

Recovery of various proteins and peptides is shown.
YMC-Pack PROTEIN-RP gives greater recovery than competitive C4 columns.

Improved durability when used with TFA solution

0.1%TFA

The graph shows the test result of the stability of the stationary phase with 0.1% aqueous TFA. Retention of diphenyl on C4 columns manufactured by other companies greatly decreases as time passes. This is caused by detachment of butyl groups from the packing material due to acid hydrolysis.
Retention of diphenyl on YMC-Pack PROTEIN-RP is shown to be stable after 500 hours of mobile phase flow.

Prior to flow&After 500 hours

Flow conditions

Eluent	: water/TFA (100/0.1)
Flow rate	: 1.0 mL/min
Temperature	: ambient

Measurement conditions

Column	: YMC-Pack PROTEIN-RP 250 x 4.6 mmI.D.
Eluent	: acetonitrile / water (40/60)
Flow rate	: 1.0 mL/min
Temperature	: 30ºC
Detection	: UV at 254 nm, 0.32AUFS