YMC-Triart Diol-HILIC 層析管柱
YMC-Triart Diol-HILIC is a HILIC (hydrophilic interaction chromatography) column based on an organic/inorganic hybrid particle synthesized with dihydroxypropyl groups. Triart Diol-HILIC is ideal for a separation of polar and hydrophilic compounds that are not retained by reversed-phase (C18, C8, and others) chromatography. Triart Diol-HILIC begins with an organic/inorganic hybrid-based particle with excellent durability and usability across a wide pH range. Excellent reproducibility is achieved by the use of the dihydroxypropyl group–a neutral functional group exhibiting low nonspecific adsorption.
SPECIFICATION
| Base | : | organic/inorganic hybrid silica |
|---|---|---|
| Particle size | : | 1.9 μm, 3 μm, 5 μm |
| Pore size | : | 120Å |
| Usable pH range | : | 2-10 |
POINT
￭ HILIC column based on an organic/inorganic hybrid silica
￭ Useful for a separation of polar and hydrophilic compounds
￭ Highly-improved chemical durability in high pH conditions
￭ Excellent versatility : wide pH range of application
￭ Excellent reproducibility : low nonspecific adsorption
Features
Triart C18 (reversed-phase) shows very weak retention and poor resolution of L-ascorbic acid and its stereoisomer (erythorbic acid), even with a 100% aqueous mobile phase. However, Triart Diol-HILIC shows strong retention and better resolution of these compounds with a mobile phase containing 90% organic solvent.
Triart Diol-HILIC provides highly reproducible separations, even at high pH and high temperatures. The lifetime of Triart Diol-HILIC is much greater than that of conventional silica-based diol columns.
| Column | ： | 5 μm, 150 X 4.6 mmI.D. |
|---|---|---|
| Eluent | ： | acetonitrile/water/NH3 (90/10/0.1) pH 11.3 |
| Temperature | ： | 50°C |
| Flow rate | ： | 1.0 mL/min |
| Sample | ： | Cytosine |
