BioPro IEX 生物樣品陰/陽離子交換樹脂純化膠體
BioPro IEX SmartSep Q/S and BioPro IEX Q/S, strong ion exchange media, are suitable for downstream chromatographic purification processes in biopharmaceutical manufacturing. High dynamic binding capacity and high recovery of those media allow fast purification processes at large scale. It offers high productivity on industrial purification.
BioPro IEX SmartSep Q/S, which show high resolution and recovery even at a high flow rate and high loading condition, are suitable for an intermediate purification step and a polishing step of MAbs, proteins, peptides, and oligonucleotides.
BioPro IEX Q/S are designed for capture and intermediate purification of biopharmaceuticals such as MAbs.
#蛋白質、抗體、寡核甘酸純化
Specification
| BioPro IEX Q/S | BioPro IEX SmartSep Q/S | |||
|---|---|---|---|---|
| BioPro IEX Q | BioPro IEX S | BioPro IEX SmartSep Q | BioPro IEX SmartSep S | |
| Matrix | Hydrophilic porous polymer | |||
| Particle size (μm) | 75 | 10, 20, 30 | ||
| Charged group | -R-N+(CH3)3 | -R-SO3– | -R-N+(CH3)3 | -R-SO3– |
| Ion-exchange capacity (meq/mL-resin) |
>0.10 |
>0.08 | ||
| Dynamic binding capacity (DBC) (mg/mL-resin) |
>160 (BSA) | >160 (lysozyme) | >100 (BSA) | >100 (lysozyme) |
Features
- • Hydrophilic polymer beads with low nonspecific adsorption
- • Suitable for purification of antibodies, proteins and nucleic acids
- • High dynamic binding capacity (DBC) for various samples
- • High DBC over a wide range of flow rates
- • High Temperature Stability
- • Excellent durability under CIP
High dynamic binding capacity (DBC) for various samples
BioPro IEX SmartSep Q/S have higher DBC than conventional ion exchange media. In particular, for IgG, the DBC of BioPro IEX SmartSep Q/S is more than twice as high as that of the competitor.
This feature of BioPro IEX SmartSep Q/S makes purification productivity of IgG per unit time double or more.
| Particle size (µm) |
DBC (mg/mL-resin, 10% breakthrough) | |||
|---|---|---|---|---|
| Insulin | Lysozyme | Human Polyclonal lgG | ||
| BioPro IEX SmartSep S30 | 30 | 73 | 111 | 93 |
| Brand T (Porous S type) | 30 | 67 | 72 | 42 |
| Brand G (Porous S type) | 30 | 64 | 85 | 41 |
High DBC over a wide range of flow rates
As the high DBC of BioPro IEX SmartSep Q/S is maintained at higher flow rates, it is suitable for high-speed purifications with 2-4 times the conventional flow rate. This feature offers a significant improvement of productivity.
High temperature stability
BioPro IEX SmartSep Q/S can be used at temperatures up to 60 °C, offering great flexibility in method development. The elution profile of an antisense oligonucleotide remained constant even after injecting the sample in 130 runs at 60 °C. There is no retention time shift and the column efficiency remains the same with BioPro IEX SmartSep Q.
Excellent durability under CIP
Cleaning in place (CIP) is an important procedure for cleaning and sterilizing columns used for protein purification. The DBC and the selectivity of proteins are unaffected following 20 cycles of CIP with 1 M NaOH. The high chemical stability of BioPro IEX Q/S allow effective cleaning with alkaline solution.
