CHIRAL ART Coated type 手性分離管柱(不對稱/鏡相)

CHIRAL ART Coated type silica packing material is coated with polysaccharide derivatives. CHIRAL ART are suitable for the separation of a wide range of chiral compounds, cis-trans isomers, and geometric isomers. CHIRAL ART are available in HPLC columns and in preparative grades, in large (multi kg) quantities.

SPECIFICATION

Base : organic/inorganic hybrid silica
Particle size : 1.9 μm, 3 µm, 5 µm
Pore size : 120 Å
Carbon contents : 15%
pH range : 1-8

Lineup

Column/Packing material Particle size (µm) Chiral selector USP Code
Amylose-C
Amylose-C Neo
3
5
10
20
L51
Cellulose-C L40
Usable mobile phase n-hexane, n-heptane, ethanol, 2-propanol, acetonitrile, etc.

Amylose-C Neo is a grade-up column/packing material of Amylose-C.

Features

CHIRAL ART provide good peak shapes on ionic and metal coordination compounds.

Spectrum1
Column 5 μm, 250 X 4.6 mmI.D.
Eluent n-hexane/2-propanol/diethylamine
(80/20/0.1)
Flow rate 1.0 mL/min
Temperature 25°C
Detection UV at 230 nm
Injection 10 μL (0.1 mg/mL)

CHIRAL ART have outstanding packed bed stability provided by the use of high-strength super-wide-pore silica and innovative packing technology. The column efficiency and selectivity are maintained, even after sequential gradient tests at a high flow rate (three times higher than the normal flow rate) and a high pressure (rapid pressure change).
CHIRAL ART are suitable for the case where one intends to shorten an analysis time, (re-)equilibration time, and/or column cleaning time by increasing the flow rate. This feature is also effective when a highly viscous solvent needs to be used as a mobile phase on immobilized type columns.

Photo1
Sequential gradient test
Column 5 μm, 250 X 4.6 mmI.D.
Eluent A) n-hexane,
B) ethanol
0-100%B (0-15 min)
Flow rate 3.0 mL/min
Pressure 10-30 MPa/run
Temperature 37℃
Column performance test
Column 5 μm, 250 X 4.6 mmI.D.
Eluent n-hexane/ethanol
(90/10)
Flow rate 1.0 mL/min
Temperature 37℃
Detection UV at 230 nm
Sample trans-Stilbene oxide
Photo2

On both analytical and preparative separation, separation behavior of CHIRAL ART is equivalent to that of the competitor’s product.

Photo3
Photo4

trans-Stilbene oxide

Column 5 μm, 250 X 4.6 mmI.D.
Eluent n-hexane/ethanol (90/10)
Flow rate 1.0 mL/min

Ibuprofen enantiomers were purified by utilizing the recycling mode of a multiple preparative HPLC system, LC-Forte/R. Recycling chromatography is effective when method optimization for chiral isolation is difficult using single column separation. The recycling method offers high-purity and high-recovery purification. Furthermore, no solvent is consumed during recycling, which greatly contributes to the reduction of solvent consumption during purification.

Photo5



		

Ibuprofen

Column CHIRAL ART Cellulose-C 10 µm, 250 X 20 mmI.D.
Flow rate 20 mL/min
Detection UV at 265 nm
Injection 5 mL (10 mg/mL)
Single Column Recycling
Enantiomeric purity (%ee )
Fr.1 (R)(- -)-Ibuprofen
Fr.2 (S)(- +)-Ibuprofen
95.0
96.8
98.4
99.2
Yield (%) 84 95
Solvent consumption
(mL solvent/g product)
9,523 1,276

Technical data