CHIRAL ART Immobilized type 手性分離管柱(不對稱/鏡相)

CHIRAL ART Immobilized type is an HPLC column with polysaccharide derivatives chiral selector. CHIRAL ART Immobilized type can be used either in normal or reversed-phase modes. CHIRAL ART are suitable for the separation of a wide range of chiral compounds, cis-trans isomers, and geometric isomers. CHIRAL ART are available in HPLC columns and in preparative grades, in large (multi kg) quantities.

Column/Packing material Particle size (µm) Chiral selector USP Code
CHIRAL ART
Amylose-SA
3
5
10
20
L99
CHIRAL ART
Cellulose-SB
CHIRAL ART
Cellulose-SC
L119
CHIRAL ART
Cellulose-SJ
CHIRAL ART
Cellulose-SZ
Usable mobile phase Normal-phase n-hexane, n-heptane, methanol, ethanol, 2-propanol, acetonitrile, ethyl acetate, tetrahydrofuran, chloroform, t-butyl methyl ether, etc.
Reversed-phase acetonitrile, methanol, ethanol, 2-propanol, tetrahydrofuran, water, aqueous buffer, etc.

-Excellent peak shape

CHIRAL ART provide good peak shapes for ionic and metal coordination compounds.

Spectrum1
Column 5 μm, 250 X 4.6 mmI.D.
Eluent n-hexane/2-propanol/diethylamine
(80/20/0.1)
Flow rate 1.0 mL/min
Temperature 25°C
Detection UV at 230 nm
Injection 10 μL (0.1 mg/mL)

-Low column bleeding

CHIRAL ART Immobilized type show remarkably reduced background signals under typical gradient conditions. The low column bleeding of these columns provides high sensitivity for LC/MS analysis owing to the very low ion suppression and stable baseline. CHIRAL ART Immobilized type offers excellent robustness for gradient analysis and highly sensitive analysis on LC/MS.

Spectrum2
Gradient test
Column 5 μm, 250 X 4.6 mmI.D.
Eluent A) n-hexane
B) ethanol
2-80%B (0-30 min)
Flow rate 1.0 mL/min
Temperature 25°C
Detection UV at 230 nm

-Wide usable pH range

CHIRAL ART Immobilized type has excellent chemical stability and can be used across a wide pH range. The addition of acid/amine to the mobile phase or the use of buffer solution is an effective tool for improving peak shape and/or resolution.

Photo1
Continuous flow of acid/alkaline solution
Column 5 μm, 50 X 4.6 mmI.D.
Eluent Buffer/methanol (90/10)
Flow rate 1.0 mL/min
【Acidic condition】
Buffer 0.1% H3PO4 (pH 2)
Temperature 40°C
【Basic condition】
Buffer 20 mM NH4HCO3-DEA (pH 9)
Temperature 25°C
Column performance test
Column 5 μm, 50 X 4.6 mmI.D.
Eluent acetonitrile/water (30/70) for SB
methanol/water (70/30) for SJ
Flow rate 1.0 mL/min
Temperature 25°C
Detection UV at 254 nm
Sample Benzoin for SB
1-(1-Naphthyl) ethanol for SJ

-High solvent versatility

On CHIRAL ART Immobilized type, the change in column performance after flushing with each solvent was less than 2%. Thus, these columns have high resistance to various solvents.

Retention rate of initial column performance (after flushing with 1,000CV of each solvent at 40°C)  *CV=Column Volume

Amylose-SA Cellulose-SB Cellulose-SJ
α k'(2) α k'(2) α k'(2)
Ethyl acetate 100.3% 101.2% 100.0% 99.1% 99.3% 99.0%
Tetrahydrofuran 100.0% 100.0% 99.3% 98.0% 99.2% 99.7%
Dichloromethane 100.3% 100.6% 101.3% 99.6% 99.6% 98.4%
Column 5 μm, 50 X 4.6 mmI.D.
Eluent n-hexane/2-propanol (95/5)
Flow rate 1.0 mL/min
Temperature 25°C
Sample Benzoin

Amylose-SA

Particle size
(µm)
Column size
Inner diameter X length (mm)
Product number
3 2.0 X 75 KSA99S03-L502WT
2.0 X 100 KSA99S03-1002WT
2.0 X 150 KSA99S03-1502WT
2.0 X 250 KSA99S03-2502WT
3.0 X 50 KSA99S03-0503WT
3.0 X 75 KSA99S03-L503WT
3.0 X 100 KSA99S03-1003WT
3.0 X 150 KSA99S03-1503WT
3.0 X 250 KSA99S03-2503WT
4.6 X 50 KSA99S03-0546WT
4.6 X 75 KSA99S03-L546WT
4.6 X 100 KSA99S03-1046WT
4.6 X 150 KSA99S03-1546WT
4.6 X 250 KSA99S03-2546WT
5 4.6 X 150 KSA99S05-1546WT
4.6 X 250 KSA99S05-2546WT
10 X 250 KSA99S05-2510WT
20 X 250 KSA99S05-2520WX
30 X 250 KSA99S05-2530WX

Cellulose-SB

Particle size
(µm)
Column size
Inner diameter X length (mm)
Product number
3 2.0 X 75 KSB99S03-L502WT
2.0 X 100 KSB99S03-1002WT
2.0 X 150 KSB99S03-1502WT
2.0 X 250 KSB99S03-2502WT
3.0 X 50 KSB99S03-0503WT
3.0 X 75 KSB99S03-L503WT
3.0 X 100 KSB99S03-1003WT
3.0 X 150 KSB99S03-1503WT
3.0 X 250 KSB99S03-2503WT
4.6 X 50 KSB99S03-0546WT
4.6 X 75 KSB99S03-L546WT
4.6 X 100 KSB99S03-1046WT
4.6 X 150 KSB99S03-1546WT
4.6 X 250 KSB99S03-2546WT
5 4.6 X 150 KSB99S05-1546WT
4.6 X 250 KSB99S05-2546WT
10 X 250 KSB99S05-2510WT
20 X 250 KSB99S05-2520WX
30 X 250 KSB99S05-2530WX

Cellulose-SC

Particle size
(µm)
Column size
Inner diameter X length (mm)
Product number
3 2.0 X 75 KSC99S03-L502WT
2.0 X 100 KSC99S03-1002WT
2.0 X 150 KSC99S03-1502WT
2.0 X 250 KSC99S03-2502WT
3.0 X 50 KSC99S03-0503WT
3.0 X 75 KSC99S03-L503WT
3.0 X 100 KSC99S03-1003WT
3.0 X 150 KSC99S03-1503WT
3.0 X 250 KSC99S03-2503WT
4.6 X 50 KSC99S03-0546WT
4.6 X 75 KSC99S03-L546WT
4.6 X 100 KSC99S03-1046WT
4.6 X 150 KSC99S03-1546WT
4.6 X 250 KSC99S03-2546WT
5 4.6 X 150 KSC99S05-1546WT
4.6 X 250 KSC99S05-2546WT
10 X 250 KSC99S05-2510WT
20 X 250 KSC99S05-2520WX
30 X 250 KSC99S05-2530WX

Cellulose-SJ

Particle size
(µm)
Column size
Inner diameter X length (mm)
Product number
3 2.0 X 75 KSJ99S03-L502WT
2.0 X 100 KSJ99S03-1002WT
2.0 X 150 KSJ99S03-1502WT
2.0 X 250 KSJ99S03-2502WT
3.0 X 50 KSJ99S03-0503WT
3.0 X 75 KSJ99S03-L503WT
3.0 X 100 KSJ99S03-1003WT
3.0 X 150 KSJ99S03-1503WT
3.0 X 250 KSJ99S03-2503WT
4.6 X 50 KSJ99S03-0546WT
4.6 X 75 KSJ99S03-L546WT
4.6 X 100 KSJ99S03-1046WT
4.6 X 150 KSJ99S03-1546WT
4.6 X 250 KSJ99S03-2546WT
5 4.6 X 150 KSJ99S05-1546WT
4.6 X 250 KSJ99S05-2546WT
10 X 250 KSJ99S05-2510WT
20 X 250 KSJ99S05-2520WX
30 X 250 KSJ99S05-2530WX

Cellulose-SZ

Particle size
(µm)
Column size
Inner diameter X length (mm)
Product number
3 2.0 X 75 KSZ99S03-L502WT
2.0 X 100 KSZ99S03-1002WT
2.0 X 150 KSZ99S03-1502WT
2.0 X 250 KSZ99S03-2502WT
3.0 X 50 KSZ99S03-0503WT
3.0 X 75 KSZ99S03-L503WT
3.0 X 100 KSZ99S03-1003WT
3.0 X 150 KSZ99S03-1503WT
3.0 X 250 KSZ99S03-2503WT
4.6 X 50 KSZ99S03-0546WT
4.6 X 75 KSZ99S03-L546WT
4.6 X 100 KSZ99S03-1046WT
4.6 X 150 KSZ99S03-1546WT
4.6 X 250 KSZ99S03-2546WT
5 4.6 X 150 KSZ99S05-1546WT
4.6 X 250 KSZ99S05-2546WT
10 X 250 KSZ99S05-2510WT
20 X 250 KSZ99S05-2520WX
30 X 250 KSZ99S05-2530WX

Amylose-SA Cartridge Columns

Particle size
(µm)
Column size
Inner diameter X length (mm)
/pack Product Number
3 2.1 X 10 5 KSA99S03-01Q1GC
1 KSA99S03-01Q1GC-1
2.1 X 20 5 KSA99S03-02Q1GC
1 KSA99S03-02Q1GC-1
3.0 X 10 5 KSA99S03-0103GC
1 KSA99S03-0103GC-1
3.0 X 20 5 KSA99S03-0203GC
1 KSA99S03-0203GC-1
4.0 X 10 5 KSA99S03-0104GC
1 KSA99S03-0104GC-1
4.0 X 20 5 KSA99S03-0204GC
1 KSA99S03-0204GC-1
5 4.0 X 10 5 KSA99S05-0104GC
1 KSA99S05-0104GC-1
4.0 X 20 5 KSA99S05-0204GC
1 KSA99S05-0204GC-1
10 X 10 2 KSA99S05-0110CC
20 X 10 2 KSA99S05-0120CCN
30 X 10 2 KSA99S05-0130CCN

*When using it for the first time, please purchase the cartridge holder corresponding to size of column.

Cellulose-SB Cartridge Columns

Particle size
(µm)
Column size
Inner diameter X length (mm)
/pack Product Number
3 2.1 X 10 5 KSB99S03-01Q1GC
1 KSB99S03-01Q1GC-1
2.1 X 20 5 KSB99S03-02Q1GC
1 KSB99S03-02Q1GC-1
3.0 X 10 5 KSB99S03-0103GC
1 KSB99S03-0103GC-1
3.0 X 20 5 KSB99S03-0203GC
1 KSB99S03-0203GC-1
4.0 X 10 5 KSB99S03-0104GC
1 KSB99S03-0104GC-1
4.0 X 20 5 KSB99S03-0204GC
1 KSB99S03-0204GC-1
5 4.0 X 10 5 KSB99S05-0104GC
1 KSB99S05-0104GC-1
4.0 X 20 5 KSB99S05-0204GC
1 KSB99S05-0204GC-1
10 X 10 2 KSB99S05-0110CC
20 X 10 2 KSB99S05-0120CCN
30 X 10 2 KSB99S05-0130CCN

*When using it for the first time, please purchase the cartridge holder corresponding to size of column.

Cellulose-SC Cartridge Columns

Particle size
(µm)
Column size
Inner diameter X length (mm)
/pack Product Number
3 2.1 X 10 5 KSC99S03-01Q1GC
1 KSC99S03-01Q1GC-1
2.1 X 20 5 KSC99S03-02Q1GC
1 KSC99S03-02Q1GC-1
3.0 X 10 5 KSC99S03-0103GC
1 KSC99S03-0103GC-1
3.0 X 20 5 KSC99S03-0203GC
1 KSC99S03-0203GC-1
4.0 X 10 5 KSC99S03-0104GC
1 KSC99S03-0104GC-1
4.0 X 20 5 KSC99S03-0204GC
1 KSC99S03-0204GC-1
5 4.0 X 10 5 KSC99S05-0104GC
1 KSC99S05-0104GC-1
4.0 X 20 5 KSC99S05-0204GC
1 KSC99S05-0204GC-1
10 X 10 2 KSC99S05-0110CC
20 X 10 2 KSC99S05-0120CCN
30 X 10 2 KSC99S05-0130CCN

*When using it for the first time, please purchase the cartridge holder corresponding to size of column.

Cellulose-SJ Cartridge Columns

Particle size
(µm)
Column size
Inner diameter X length (mm)
/pack Product Number
3 2.1 X 10 5 KSJ99S03-01Q1GC
1 KSJ99S03-01Q1GC-1
2.1 X 20 5 KSJ99S03-02Q1GC
1 KSJ99S03-02Q1GC-1
3.0 X 10 5 KSJ99S03-0103GC
1 KSJ99S03-0103GC-1
3.0 X 20 5 KSJ99S03-0203GC
1 KSJ99S03-0203GC-1
4.0 X 10 5 KSJ99S03-0104GC
1 KSJ99S03-0104GC-1
4.0 X 20 5 KSJ99S03-0204GC
1 KSJ99S03-0204GC-1
5 4.0 X 10 5 KSJ99S05-0104GC
1 KSJ99S05-0104GC-1
4.0 X 20 5 KSJ99S05-0204GC
1 KSJ99S05-0204GC-1
10 X 10 2 KSJ99S05-0110CC
20 X 10 2 KSJ99S05-0120CCN
30 X 10 2 KSJ99S05-0130CCN

*When using it for the first time, please purchase the cartridge holder corresponding to size of column.

Cellulose-SZ Cartridge Columns

Particle size
(µm)
Column size
Inner diameter X length (mm)
/pack Product Number
3 2.1 X 10 5 KSZ99S03-01Q1GC
1 KSZ99S03-01Q1GC-1
2.1 X 20 5 KSZ99S03-02Q1GC
1 KSZ99S03-02Q1GC-1
3.0 X 10 5 KSZ99S03-0103GC
1 KSZ99S03-0103GC-1
3.0 X 20 5 KSZ99S03-0203GC
1 KSZ99S03-0203GC-1
4.0 X 10 5 KSZ99S03-0104GC
1 KSZ99S03-0104GC-1
4.0 X 20 5 KSZ99S03-0204GC
1 KSZ99S03-0204GC-1
5 4.0 X 10 5 KSZ99S05-0104GC
1 KSZ99S05-0104GC-1
4.0 X 20 5 KSZ99S05-0204GC
1 KSZ99S05-0204GC-1
10 X 10 2 KSZ99S05-0110CC
20 X 10 2 KSZ99S05-0120CCN
30 X 10 2 KSZ99S05-0130CCN

*When using it for the first time, please purchase the cartridge holder corresponding to size of column.

Cartridge Holder

Product Number
Cartridge holder
(Inner diameter: 1.0-4.0 mm)
length: 10 mm XPGCHP1
length: 20 mm XPGCHP2
Semi-prep Cartridge Holder Inner diameter: 10 mm XPCHSPW1
Inner diameter: 20 mm XPGHFSP20ID
Inner diameter: 30 mm XPGHFSP30ID

Packing Materials

Packing material Particle size
(µm)
Product number
Amylose-SA 5 KSA99S05
10 KSA99S11
20 KSA99S21
Cellulose-SB 5 KSB99S05
10 KSB99S11
20 KSB99S21
Cellulose-SC 5 KSC99S05
10 KSC99S11
20 KSC99S21
Cellulose-SJ 5 KSJ99S05
10 KSJ99S11
20 KSJ99S21