連續式層析純化系統 Continuous Chromatography
Liquid chromatography with a multi-column switching technique offers a continuous separation/purification. Such a continuous chromatography exhibits significant advantages over the traditional single-column chromatography.
These advantages include, for examples:
– Minimal consumption of affinity resins for capture of antibodies
– Time- and space-saving for purification of biopharmaceuticals
– Minimal product waste from the crude materials
YMC offers continuous LC systems both for lab-scale purification and for pilot/industrial-scale (GMP-compliant) purification. The proprietary continuous chromatography, enabling high-productivity with both of high-purity and high-yield in ion-exchange and reversed-phase chromatography (not to mention affinity chromatography), results in an excellent cost reduction for the purification of biopharmaceuticals, peptides and oligonucleotides. Our continuous chromatography systems have a simple configuration due to the twin-column switching, leading to easy understanding of the operational design. In addition, such twin-column systems allow easier maintenance than the triple- or more multi-column systems.
Comparison among systems
| Purity and Yield | Buffer consumption | Maintenance | Features | |
|---|---|---|---|---|
| Traditional chromatography (single-column) |
△ | △ | ◎ | – Theoretical operation →Trade-off between purity and yield |
| Continous chromatography (triple- or more multi-columns) |
◎ | ◎ | △ | – Improvement of affinity resin efficiency – Complicated system configuration → Hard to understand the operational design |
| Continous chromatography (twin-columns): Our systems |
◎ | ◎ | ◎ | – Unique twin-column processes achiving the combination of high-purity and high-yield – Simple system configuration → Easy to understand the operational design |
Unique continuous purification processes
Our twin-column chromatography systems offer the cost reduction including time-saving for purification of various substances such as antibodies, proteins/peptides, oligonucleotides, small molecules and the impurities in therapeutic products.
Applications
IEX purification of oligonucleotide with MCSGP.
1.5 times yield compared to single column batch purification. (56%→92%)
RP purification of peptide with MCSGP.
1.3 times yield compared to single column batch purification. (71%→92%)
IEX purification of monoclonal antibody isoforms with N-Rich.
10 times faster than single column batch purification.
Affinity purification of monoclonal antibody with CaptureSMB.
3 times efficiency compared to single column batch purification.
