MacroSep IEX Q 大孔型離子交換樹脂純化膠體
MacroSep IEX Q are innovative strong anion exchange media to target large biomolecules and particles such as adeno-associated viruses (AAVs), protein complexes, and plasmid DNA (pDNA). This media offers an optimized macro-porous structure that ensures efficient purification of these molecules. With its high binding capacity for large particles and molecules in addition to high separation efficiency even at elevated flow rates, MacroSep IEX Q is an ideal solution for downstream processing (DSP) platforms, enabling successful and productive purification of large biotherapeutics.
#蛋白質、病毒、抗體、寡核甘酸純化
MacroSep IEX Q
BioPro IEX SmartSep Q30
(meq/mL-resin)
(mg/mL-resin)
- Features
-
- • Ion exchange media based on hydrophilic polymer with macro-porous structure
- • Designed for separation of large-sized biomolecules such as AAVs and pDNA
- High resolution even at a high flow rate
- Highly efficient purification of AAV2 with MacroSep IEX Q
- The separation performance for topological isomers of pDNA
- High dynamic binding capacity (DBC) and high recovery of pDNA
High resolution even at a high flow rate
The direct comparison of MacroSep IEX Q with conventional media at two different flow rates clearly shows that MacroSep IEX Q provides excellent resolution, even at high flow rates. This leads to high productivity.
B) 20 mM Bis-tris propane-HCl containing 0.5 M TMAC* (pH 9.0)
* tetramethylammonium chloride
This research was supported by AMED under Grant Number JP18ae0201001.
Highly efficient purification of AAV2 with MacroSep IEX Q
AAV Full/Empty capsids separation was performed with MacroSep IEX Q. Purity of the full AAV2 after the chromatographic purification was reached to 84.9% against 20.5% of a feed solution, determined by analytical ultracentrifugation (AUC). MacroSep IEX Q is suitable for chromatographic purification of AAV.
B) 20 mM Bis-tris propane-HCl containing 0.5 M CC* (pH 9.0) ,1% sucrose, 0.1% poloxamer 188
20-50%B (33.75 CV)
* choline chloride
This research was supported by AMED under Grant Number JP18ae0201001.
The separation performance for topological isomers of pDNA
MacroSep IEX Q can separate supercoiled (SC) from the non-functional linearized (L) and nicked open-circular (OC) species with higher resolution while conventional media gives poor resolution of those species. MacroSep IEX offers increased productivity with higher purity on pDNA purification.
B) 20 mM Tris-HCl (pH 8.5) containing 1.0 M NaCl
82%B (0-1.5 min), 82-86%B (1.5-22.5 min)
[Brand A]
77%B (0-1.5 min), 77-81%B (1.5-22.5 min)
[OC] pUC19 plasmid digested with nicking endonuclease Nt.BspQΙ
[L] pUC19 plasmid digested with restriction enzyme BamHΙ
High dynamic binding capacity (DBC) and high recovery of pDNA
MacroSep IEX Q is designed to have higher DBC value of large biomolecules than conventional media for improving productivity. DBC value of pDNA with each MacroSep IEX Q and conventional media is determined as an example of a large-sized modality. MacroSep IEX Q shows higher DBC with high recovery, compared to the conventional media.
Breakthrough curves
26 X 7 mmI.D. (1 mL)
[Brand C]
20 X 8 mmI.D. (1 mL)
Comparisons of DBC and recovery for pDNA
| DBC (mg/mL-resin, 10% breakthrough) |
Recovery* (%) |
|
|---|---|---|
| MacroSep IEX Q | 7.5 | 101 |
| Brand C | 1.3 | 101 |
*Recovery: (Eluted amount/Adsorption amount) X 100
Column
| Column size inner diameter x length (mm) |
Particle size (µm) |
Column Volume | Product number |
|---|---|---|---|
| 5.0 X 50 | 30 | 1 mL | QM99S30-0505AT |
| 11.3 X 50 | 5 mL | QM99S30-0511AT |
Media
| Particle size (µm) |
Product number |
|---|---|
| 30 | QM99S30 |
Screening Kit
| Particle size (µm) |
Column Volume | Pack | Product number |
|---|---|---|---|
| 30 | 1 mL | 5 | BPQM99S30-01PK |
| 5 mL | BPQM99S30-05PK |
