YMC CHIRAL PREP CD ST/PM 手性分離管柱(不對稱/鏡相)

YMC CHIRAL PREP CD ST/PM are suitable packing materials for preparative isolation of optical isomers. ST is suitable for use mainly in reversed-phase mode, while PM can be used in both normal-phase and reversed-phase modes. The cost is considerably less than that of the conventional packing materials for optical isomers, and therefore YMC CHIRAL PREP CD ST/PM are appropriate not only for semi-preparative isolation but also for industrial isolation. They are also applicable to simulated moving bed(SMB) chromatography and axial compression columns.

SPECIFICATION

Particle size : 10µm,  20µm,
50µm
Pore size : 120Å
Usable pH range : 2.0-7.0

POINT

  • β-cyclodextrin modified silica gel packing material
  • Can separate various optical isomers
  • Can be used in both normal-phase and reversed-phase modes
  • Applicable to large-scale isolation by simulated moving bed(SMB) chromatography
  • Various particle sizes are available
  • Bulk supply at kilogram scale is available
  • Excellent cost performance

Features

As the differences in the hydrophobicities of the cis-trans isomers of piperine, which is a pungent component of pepper, are small, commonly used reversed phase columns are not suitable for their separation. However, YMC-Triart PFP provides excellent separations as it recognizes minor charge localizations in a molecule owing to various binding through as π–π and dipole–dipole interactions. YMC-Triart PFP shows high selectivity towards compounds with small structural differences.

Colomn:YMC CHIRAL PREP CD ST(10µm, 120Å)

Scale up from analytical to preparative conditions is fast and easy with high sample loading.

Optical isomer Column Load* Optical purity(F1) Recovery amount(F1) Recovery rate(F1)
1. PM 100mg >99.9% 49.6mg 99.2%
2. ST 100mg >99.9% 47.7mg 94.8%
3. ST 100mg >99.9% 38.5mg 77.0%
4. PM 200mg >99.9% 96.5mg 96.5%
5. PM 250mg >99.9% 114.2mg 91.3%

*Column size :250X20mm I.D. (10µm, 120Å)

Highly pure optical isomers can be obtained with high recovery.

Column : YMC CHIRAL PREP CD
PM (10µm,120Å)
250X4.6 mm I.D.
Eluent : hexane
/ethanol (95/5)
Flow rate : 0.5mL/min
Temperature : ambient
Detection : UV at 254nm
Injection : 5µL (1mg/mL)
Column : YMC CHIRAL PREP CD
ST (0µm,120Å)
250X4.6 mm I.D.
Eluent : 20mM KH2PO4 (pH4.6)
/acetonitrile (99/1)
Flow rate : 1.0mL/min
Temperature : ambient
Detection : UV at 220nm
Injection : 5µL (1mg/mL)

Besides water, alcohol or acetonitrile, hexane or tetrahydrofuran can be used as mobile phase.