YMC-Pack PVA-Sil 層析管柱
YMC-Pack PVA-Sil, bonded with a monomolecular polymer coating of vinyl alcohol (PVA), completely covers both external and internal surfaces of the silica support, protecting it against aggressive, high pH buffers and solvents. The PVA polymer shell on PVA-Sil deactivates the silica support while roviding a hydrophilic surface.
SPECIFICATION
| Particle size | : 5 µm |
|---|---|
| Pore size | : 120 Å |
| Usable pH range | : 2.0-9.5 |
| Recommended pH range | : 2.0-7.5 |
POINT
￭ Vinyl alcohol polymerised silica ￭ High stability and reproducibility ￭ Excellent choice for SFC (Supercritical Fluid Chromatography)
Features
Potato Lipids
Sample:
| S : Sterols | CER : Cerebrosides |
| SE : Sterol Esters | SG : Steryl glycosides |
| MGDG : Monogalactosyldiacylglycerols | DGDG : Digalactosyldiacylglycerols |
| PE : Phosphatidylethanolamine | PG : Phosphatidyl glycerols |
| PC : Phosphatidylcholine | ASG : Acylsterylglycosides |
| PI : Phosphatidylinositol | DG : Diacylglycerol |
(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 – 100)
| Column | : | YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D. | ||||||||||||||||||||||||||||||||||||||||||||
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| Flow rate | : | 1 to 2 mL/min | ||||||||||||||||||||||||||||||||||||||||||||
| Eluent | : | A) 2-methylpentane/methyl tert-butyl ether (98/2) B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025) C) 2-propanol/water/triethylamine (50/50/0.2) |
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| Gradient | : |
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| Nebuliser temperature | : | 25ºC, Evaporation temperature : 35ºC | ||||||||||||||||||||||||||||||||||||||||||||
| Detector | : | ELSD | ||||||||||||||||||||||||||||||||||||||||||||
Nucleotides (in HILIC mode)
1. 5'-TMP 2. 5'-UMP 3. 5'-IMP 4. 5'-AMP 5. 5'-GMP 6. 5'-CMP
