YMC*GEL/YMC*GEL HG/YMC-Omega 管柱層析純化矽膠
YMC*GEL for preparative HPLC has various available functional groups, such as reversed-phase and normal-phase, and also various particle sizes from 3 µm with high-resolution to 150 µm for open column chromatography. YMC*GEL is able to work for a long period of time without particle breakage, as it uses high-density and high-strength silica gel. YMC*GEL/YMC*GEL HG packing materials are widely used both domestically and internationally.
管柱層析純化用球型矽膠(silica gel)
POINT
￭ Packing materials with various functional groups and particle sizes are available
￭ High density and high mechanical strength silica gel (YMC*GEL HG)
￭ Suited for large-scale purification by using dynamic axial compression column
￭ Packing material for open column chromatography is available
Features
The high mechanical stability of YMC*GEL HG is demonstrated by means of repeated measurements with a dynamic axial compression column (DAC). Even after more than 10 repacking cycles with the same material, the pressure does not increase. The absence of fines is proven by a constant backpressure.
| Packing conditions | |
| Column size | 100 X 50 mmI.D. |
|---|---|
| Packing pressure | 6.5 MPa |
| Test conditions | |
| Eluent | methanol/water（85/15） |
| Flow rate | 50 mL/min |
YMC*GEL/YMC*GEL HG are available with various functional groups and also with wide pores. Therefore, this material is suitable for the preparation of peptides, such as insulin and proteins. YMC*GEL HG can make scaling up from analytical scale to preparative scale easy, as it has the same performance as that of YMC-Pack.
| Analytical condition (1), (3) | |
| Column | YMC-Pack C8 (5 µm, 200 Å) 150 X 4.6 mmI.D. |
|---|---|
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 27%B (0-5 min) 27-40%B (5-20 min) |
| Flow rate | 0.8 mL/min |
| Temperature | ambient |
| Detection | UV at 220 nm |
| Preparative condition (2) | |
| Column | YMC*GEL C8 (10 µm, 200 Å)150 X 50 mmI.D. |
|---|---|
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 27%B (0-5 min) 27-40%B (5-20 min) |
| Flow rate | 100 mL/min |
| Temperature | ambient |
| Detection | UV at 220 nm |
Optimization of the flow rate and sample loading for high yield was performed by using a preliminary column with 10 mmI.D., and the preparative column was scaled up by using dynamic axial compression columns with 200 mmI.D. Although using two times the linear flow rate slightly decreases the yield per preparation, the productivity can be increased because the preparation time per preparation is halved and the productivity per unit time increases 180%.
| Column | YMC-Omega (10 µm) |
|---|---|
| Eluent | methanol |
| Temperature | ambient |
| Detector | UV at 210 nm |
| Sample | 70% EPA-EE |
Lineup
YMC＊GEL/YMC＊GEL HG
| Packing material | Particle size (µm) |
Pore size (Å) |
Characteristics |
|---|---|---|---|
| ODS-A | 3, 5 | 120, 200, 300 | Standard ODS |
| ODS-A-HG | 10, 15, 20, 50 | ||
| ODS-AM | 3, 5 | 120 | Outstanding lot-to-lot reproducibility |
| ODS-AQ | 3, 5 | 120, 200 | Superior separation of hydrophilic compounds |
| ODS-AQ-HG | 10, 15, 20, 50 | ||
| C8 | 3, 5 | 120, 200, 300 | Useful for separation of relatively highly hydrophobic compounds
Useful for separation of proteins and peptides |
| C8-HG | 10, 15, 20, 50 | ||
| C4 | 3, 5 | 120, 200, 300 | Stationary phase with low hydrophobicity
Useful for separation of proteins and peptides |
| C4-HG | 10, 15, 20, 50 | ||
| TMS | 3, 5 | 120 | Reverse phased packing material with the lowest hydrophobicity |
| TMS-HG | 10, 15, 20, 50 | ||
| Ph(Phenyl) | 3, 5 | 120 | Different separation characteristics from C18 |
| Ph-HG | 10, 15, 20, 50 | ||
| CN | 3, 5 | 120, 300 | The medium polarity of the functional group allows selectable normal-phase and reversed-phase separation modes |
| CN-HG | 10, 15, 20, 50 | ||
| YMCbasic | 3, 5, 10 | – | Superior separation of basic compounds and peptides |
| SIL | 3, 5 | 120, 200, 300 | Standard of normal-phase packing material |
| SIL-HG | 10, 15, 20, 50 | ||
| Diol | 5 | 120, 200, 300 | Useful for gel filtration or normal-phase applications |
| Diol-HG | 10, 15, 20, 50 | ||
| NH2 | 5 | 120 | Chemically bonded with aminopropyl groups |
| NH2-HG | 10, 15, 20, 50 |
YMC-Omega
| Packing material | Particle size (µm) |
Pore size (Å) |
Characteristics |
|---|---|---|---|
| Omega | 10, 20, 50 | – | Superior separation of omega-fatty acids |
Packing materials for Open column chromatography
| Packing material | Particle size (µm) |
Pore size (Å) |
|---|---|---|
| ODS-A | 75, 150 | 120 |
| SIL | 75, 150 | 120 |
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