近年來發現PFAS可能會殘留於人類的血液以及器官中，我們摘錄了發表文獻中使用YMC Triart C18管柱結合online-SPE LC-MS/MS之檢測方法。
使用陽離子交換樹脂管柱(Cation Exchange Column)成功分析聚乙二醇修飾蛋白質(PEGylated Protein)之電荷異構物(Charge-Variant)
Polyethylene glycol(PEG)因其生物相容之特性，廣泛應用於藥物、生物醫藥及產業領域。常見於胜肽、蛋白質及其他治療藥物之應用。對於PEGylated protein之特性分析可能非常複雜並且需要使用各種不同的方法。
YMC於美國與Pelican Expression Technology實驗室合作開發出使用YMC-BioPro陽離子交換樹脂管柱進行PEGylated protein charge-variant之應用，展現出YMC BioPro管柱為此應用提供一穩定且再線性佳之分析工具，並優於目前分析charge-variant之方法。
更多詳細內容請參考LCGC雜誌內容：
The Utility of Cation Exchange Column for Successful Charge-Variant Analysis of PEGylated Proteins
使用BioPro IEX純化單株抗體(monoclonal antibodies)獲得卓越的純化效果
單株抗體(Monoclonal antibodies)是具有許多治療應用的重要生物製藥。並需要使用高性能之樹脂來有效純化這些生物分子。最佳化的樹脂對各種參數（如 pH 值或線速度linear velocity）最佳化後顯示出高結合能力。
此應用說明展示了 BioPro IEX SmartSep 在純化單克隆抗體方面的優勢。在本研究中，針對不同參數測試了不同材料。
結論：YMC BioPro IEX 為抗體純化提供了充分的靈活性(full flexibility)和高生產力(high productivity)！
作者: YMC Europe Thomas Kerkewitz/譯 YMC Taiwan
更多關於BioPro產品資訊請至以下連結，或與我們聯繫:
http://ymctaiwan.com/products/biopro_series/
新產品發表：UHPLC-MS層析管柱內徑1.0mm YMC-Triart C18
使用UHPLC進行高靈敏度分析的需求逐漸增加，因此我們提供了更窄的層析管柱，使客戶得到更好的分離結果。
更多資訊請來信索取(info@ymctaiwan.com)。
自填式管柱(self-packed columns)之品質控制
填充管柱後，建議在進行樣品純化之前，使用標準樣品進行理論塔板數（N, number of theoretical plates）、理論塔板高度（HETP, height equivalent to a theoretical plate）和峰不對稱因子（As, peak asymmetry factor）測試及計算。藉由定期和重複執行此類標準測試，可以有效地評估膠體(gel, packing material)的品質和耐用性。 如果您想比較各個測試之間的結果，建議在每次測試中在相同條件下使用相同的測量設備。測量結果可能會受到一些因素影響，例如：死體積（dead volume）、流速（flow rate）、溫度（temperature）、沖提液（eluent）和進樣量（sample loading amount）的差異。
下列公式可用於計算理論塔板數（N, number of theoretical plates）。 較大的 N 值對應於層析管柱中較高的填充膠體密度。 此外，在固定滯留時間（retention time），峰形越窄，N 值越高；柱長越長，粒徑越小，N 值越大。 通常，每柱床長（m）的理論塔板數通常用作評估層析管柱純化性能的指標。
N = 5.54 x (tR / W0.5h)2
N/m ＝ N / L
L ＝ Column bed length (m)
理論塔板高度 （HETP, height equivalent to a theoretical plate）有時也用作評估層析管柱性能的指標。 下列公式可用於計算 HETP。 該值越小，填充在柱中的膠體密度越高。
HETP ＝ L / N
洗脫峰形 （peak shape）也是評估層析管柱性能的重要因素。 峰不對稱因子 (As) 的測定是一種相對簡單的估計層析管柱性能的方法。 下列公式可用於計算 As，該值越接近“1”是越理想的，其表示越高的峰形對稱性。
As ＝ b / a
HPLC技術指引：管柱平衡(Column Equilibration)
我應該平衡HPLC管柱多久時間呢？
在執行梯度條件後進行下一次進樣前之HPLC層析管柱平衡時，如果（重新）平衡時間太短，則會出現實驗重複性不佳的風險。如果時間太長，則會浪費時間和溶劑。
在本HPLC技術指引中，您將學習如何通過計算管柱體積來估算最佳平衡體積。此外，還使用了一個實際案例來顯示不同平衡體積對分析結果的影響。有關管柱平衡的詳細說明，您可以在此處下載HPLC技術指引。
Expert Tip: 管柱再生Column regeneration
您的(U)HPLC層析管柱之分離效果是否開始變差？
這份文件我們提供了合適的管柱清洗程序，有助於恢復層析管柱之性能。此外，該程序還可防止污染物在固定相上累積。但是，需考量一些情況，例如：清潔溶劑的類型和有效再生所需的體積。有關層析管柱再生的更多詳細信息，請在此處下載說明文件。
Improving the retention behaviour of hydrophilic compounds on polar stationary phases
Do you analyse hydrophilic compounds? Do you want to improve the retention on polar stationary phases such as PFP or Diol?
In this technical note, two different options for the improved analysis of hydrophilic compounds on YMC-Triart PFP and YMC-Triart Diol-HILIC are shown. The pH value and the amount of organic solvent are examined to learn more about the retention behaviour of basic, acidic and zwitterionic compounds using these phases. Due to their different selectivity, pentafluorophenyl (PFP) or Diol modifications are an ideal choice for the analysis of hydrophilic compounds which often remain unretained or unresolved on conventional C18 phases.
You can download the full method details here.
To get further information about the YMC-Triart series of columns.
Continuous Manufacturing & Analytics Webinar
Register today to attend Continuous Manufacturing & Analytics, our free-to-attend webinar series featuring novel technologies and advanced analytics that are enabling the implementation, optimization and scale up of intensified and fully-continuous processing.
Register: Continuous Manufacturing & Analytics Webinar
DAY 4 – JUNE 24, 2021
YMC Breakout Sessions:
First Session: Continuous Integrated Production of Therapeutic Proteins: The Role of Digitalization
The important role of digitization for advancing the implementation of continuous processing to the production of therapeutic proteins will be outlined by Prof Morbidelli
Speaker
Professor Massimo Morbidelli
Politecnico Milano
Second Session: Continuous Chromatography of Peptides: A CMO Perspective
The current modus operandi for the downstream processing of synthetic peptides is reversed phase chromatography performed in batch mode. Continuous chromatography is expected to be a disruptive technology for this very cost intensive part of synthetic peptide manufacturing. Results from a proof of principle study will be presented and compared to traditional batch chromatography
Speaker:
Dr. Ralf Eisenhuth
Process Manager Chromatography and Technology Transfer
Bachem
Third Session: How Certain Resins Further Accelerate MCC Gains
Speaker:
Dr. Hans Johansson
Global Application Director
Purolite Life Sciences.
Fourth Session: UV Based in Line Control of Twin-Column Continuous Countercurrent Chromatography; AutoPeak and AutomAb
The in-line control during the application of twin-column continuous countercurrent chromatography is crucial to optimize the yield during extended / continuous runs. Changing conditions such as degradation of Protein A resin (fouling) and / or titer of feedstock must be accounted for in the control scheme of multi-column chromatography. A patented in-line UV arrangement applied to either LPLC or HPLC versions of a twin-column system can automatically adjust operation “on-the-fly” without intimate operator intervention based on the user applied risk tolerance.
Speaker:
Thomas Müller-Späth, Ph.D
Chief Technical Officer
YMC ChromaCon AG
Fast UHPLC Analysis of SARS-CoV-2 drug Remdesivir and its active metabolite GS-441524
As the COVID-19 pandemic continues, the worldwide search for an effective treatment is still ongoing. Remdesivir and its active metabolite GS-441524 are both antiviral drugs that have been examined and partially approved for the treatment of COVID-19 due to their capability of inhibiting virus replication.
In this application note, the separation of GS-441524 and Remdesivir in only 3 minutes is shown. A YMC-Triart C18 UHPLC column provides fast and reliable results making it an ideal choice for QC purposes.
You can download the application note with the full method details here.
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