Sphingolipids are major components forming biological membranes, and they serve as intracellular signaling molecules. It is important to measure the amount of these molecules in biological samples because it greatly influences on various metabolic diseases such as obesity, diabetes, and Alzheimer’s disease. However, on the LC analysis of sphingophospholipids, such as sphingosine-1-phosphate (S1P) and ceramide-1-phosphate (C1P), a phosphate group in those molecules causes significant peak tailing, and thus loss of sensitivity and reproducibility. Recently, the improved method using YMC-Triart C18 metal free column was reported by Dr. Gowda et al. 1)
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