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Improving the retention behaviour of hydrophilic compounds on polar stationary phases
Do you analyse hydrophilic compounds? Do you want to improve the retention on polar stationary phases such as PFP or Diol?
In this technical note, two different options for the improved analysis of hydrophilic compounds on YMC-Triart PFP and YMC-Triart Diol-HILIC are shown. The pH value and the amount of organic solvent are examined to learn more about the retention behaviour of basic, acidic and zwitterionic compounds using these phases. Due to their different selectivity, pentafluorophenyl (PFP) or Diol modifications are an ideal choice for the analysis of hydrophilic compounds which often remain unretained or unresolved on conventional C18 phases.
You can download the full method details here.
To get further information about the YMC-Triart series of columns.
Continuous Manufacturing & Analytics Webinar
Register today to attend Continuous Manufacturing & Analytics, our free-to-attend webinar series featuring novel technologies and advanced analytics that are enabling the implementation, optimization and scale up of intensified and fully-continuous processing.
Register: Continuous Manufacturing & Analytics Webinar
DAY 4 – JUNE 24, 2021
YMC Breakout Sessions:
First Session: Continuous Integrated Production of Therapeutic Proteins: The Role of Digitalization
The important role of digitization for advancing the implementation of continuous processing to the production of therapeutic proteins will be outlined by Prof Morbidelli
Speaker
Professor Massimo Morbidelli
Politecnico Milano
Second Session: Continuous Chromatography of Peptides: A CMO Perspective
The current modus operandi for the downstream processing of synthetic peptides is reversed phase chromatography performed in batch mode. Continuous chromatography is expected to be a disruptive technology for this very cost intensive part of synthetic peptide manufacturing. Results from a proof of principle study will be presented and compared to traditional batch chromatography
Speaker:
Dr. Ralf Eisenhuth
Process Manager Chromatography and Technology Transfer
Bachem
Third Session: How Certain Resins Further Accelerate MCC Gains
Speaker:
Dr. Hans Johansson
Global Application Director
Purolite Life Sciences.
Fourth Session: UV Based in Line Control of Twin-Column Continuous Countercurrent Chromatography; AutoPeak and AutomAb
The in-line control during the application of twin-column continuous countercurrent chromatography is crucial to optimize the yield during extended / continuous runs. Changing conditions such as degradation of Protein A resin (fouling) and / or titer of feedstock must be accounted for in the control scheme of multi-column chromatography. A patented in-line UV arrangement applied to either LPLC or HPLC versions of a twin-column system can automatically adjust operation “on-the-fly” without intimate operator intervention based on the user applied risk tolerance.
Speaker:
Thomas Müller-Späth, Ph.D
Chief Technical Officer
YMC ChromaCon AG
Fast UHPLC Analysis of SARS-CoV-2 drug Remdesivir and its active metabolite GS-441524
As the COVID-19 pandemic continues, the worldwide search for an effective treatment is still ongoing. Remdesivir and its active metabolite GS-441524 are both antiviral drugs that have been examined and partially approved for the treatment of COVID-19 due to their capability of inhibiting virus replication.
In this application note, the separation of GS-441524 and Remdesivir in only 3 minutes is shown. A YMC-Triart C18 UHPLC column provides fast and reliable results making it an ideal choice for QC purposes.
You can download the application note with the full method details here.
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