YMC-Triart C18 逆相層析管柱

YMC-Triart C18液相層析管柱採用有機雜化矽膠(hybrid silica-based)為基質,提升耐用性、低操作壓力及出色的分離能力,可在廣泛之pH值和溫度範圍下操作。C18官能基的密度經過最佳化修飾,可實現適度的疏水性和氫鍵鍵合,因此可作為各種樣品分離首選之層析管柱,即使在100%的水流動相條件下也可獲得良好的保留和再現性。

Triart C18產品介紹PDF

Triart材質介紹

用於UHPLC-MS之1.0mm管柱目錄

Metal-Free無金屬接觸材質管柱

Accura bioinert生物惰性材質管柱

SPECIFICATION
Base organic/inorganic hybrid silica
Particle size 1.9 μm, 3 μm, 5 μm
Pore size 12 nm
Carbon contents 20%
Usable pH range 1-12
USP L1

技術資料

提供方法開發上管柱之靈活運用性-YMC-Triart C18 可應用於廣泛之pH範圍以及可於高溫下操作,可提供各領域LC分析專家更靈活地條件開發,最佳化待測物分離度及滯留時間。
高靈敏度之 LC/MS應用-Highly sensitive LC/MS analysis of “tetracycline antibiotics” and “triazolam and etizolam”
Analysis of peptides and proteins
Analysis of hinokitiol
Analysis of organic acids
Analysis of aromatic carboxylic acids
Analysis of basic drugs
Fast UHPLC analyses of small peptides, Glyphosate and AMPA. According to DIN ISO 16308. 

快速UHPLC Cell Penetrating Peptides分析(Octaarginine, HIV-1 Tat Protein, Penetratin)

-Long column lifetime under harsh chemical conditions

YMC-Triart C18 offers outstanding chemical and physical durability over a wide pH range at high temperatures.
As erythromycins are known to be easily degraded under acidic (pH < 6.5) conditions, higher pH is preferable. In addition, higher temperatures tend to show better peak shape. The enhanced chemical durability of Triart C18 enables highly reproducible analysis at high pH and high temperature.

Column YMC-Triart C18 (3 μm, 120Å), 50 X 2.0 mmI.D.
Eluent 20 mM KH2PO4-K2HPO4 (pH 7.9)/acetonitrile/methanol (40/45/15)
Flow rate 0.2 mL/min
Temperature 70ºC
Detection UV at 210 nm

-Effective for analysis of highly polar compounds using 100% aqueous conditions

Using 100% aqueous mobile phase with conventional C18 columns generally shows poor performance (retention and peak shape) owing to low surface hydration caused by repulsion between aqueous mobile phase and the hydrophobic bonded phase. Triart C18 is designed to retain both moderate hydrogen bonding capacity and hydrophobicity on the surface by optimizing the bonding density of the C18 phase. Its versatility makes it ideal for a first choice ODS column, and is also applicable to the analysis of polar compounds under 100% aqueous mobile phase conditions.
In the experiment illustrated here, Triart C18 shows stable retention of organic acids, even after stopping the pump.

1. Oxalic acid
2. Tartaric acid
3. Glycolic acid
4. Formic acid
5. L-Malic acid
6. Malonic acid
7. Lactic acid

8. Acetic acid
9. Maleic acid
10 . Citric acid
11 . Succinic acid
12 . Fumaric acid
13 . Acrylic acid
14 . Propionic acid

Column YMC-Triart C18 (3 μm, 120 Å), 150 X 3.0 mmI.D.
Eluent 20 mM H3PO4
Flow rate 0.425 mL/min
Temperature 37ºC
Detection UV at 220 nm

-Minimizing strong solvent/sample loading effects

YMC-Triart C18 can tolerate larger injection volumes of samples containing solvents that have a strong eluting ability (e.g., acetonitrile), while giving better peak shapes than conventional columns. This can be important for samples pre-treated with higher concentrations of organic solvent, crude reaction samples, and samples with poor solubility.

Influence of injection volume on peak shape

Column 5 μm, 50 X 2.0 mmI.D. or 2.1 mmI.D.
Eluent A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5%B (0-0.5 min), 5-100%B (0.5-2.5 min)
Flow rate 0.4 mL/min
Temperature 40ºC
Detection UV at 275 nm

-Ideal for high-sensitivity analysis using LC/MS

On the Triart C18 column, very low levels of bleeding (leaching) are achieved due to improvements of the production procedure and product stability. The background noise of Triart C18 on LC/MS (TIC) is almost the same as that of a blank run with no column. These results prove that there is little bleeding from the Triart C18 column. Very low background noise and a high S/N ratio are expected on Triart columns, even with high-sensitivity detectors.

Bleed test by LC/MS

Column 5 µm, 50 X 2.0 mmI.D.
Eluent A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5%B (0-1 min), 5-100%B (1-5 min), 100%B (5-10 min), 100-5%B (10-10.1 min), 5%B (10.1-12.5 min)
Flow rate 0.4 mL/min
Temperature 40ºC
Detection ESI positive, TIC (Mass Range: 50-1000)

-For separation of biomolecules with molecular weight of up to 30,000 using high temperature

Changing the column temperature will provide selectivity change as well as peak shape improvement. YMC-Triart C18, which offers excellent durability even under an elevated temperature condition, is effective for the separation of peptides and proteins.

  1. Leu-Enkephalin    (MW 556)
  2. Oxytocin        (MW 1,007)
  3. β-Endorphin       (MW 3,465)
  4. Insulin         (MW 5,733)
  5. β-Lactoglobulin A (MW 18,400)
Column YMC-Triart C18 (1.9 μm, 120 Å), 50 X 2.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
10-80%B (0-5 min)
Flow rate 0.4 mL/min
Detection 220 nm
Application Columns Compound name
7 THC Related Compounds on YMC-Triart C18 YMC-Triart C18 Cannabidiolic acid Cannabigerol Cannabidiol Cannabinol Δ9-Tetrahydrocannabinol Δ8-Tetrahydrocannabinol Tetrahydrocannabinolic acid Download
ATP and its related compounds in Tuna meat YMC-Triart C18 5′-IMP 5′-ATP 5′-ADP 5′-AMP Hypoxanthine Inosine Download
Aciclovir for syrup YMC-Triart C18 Aciclovir (Acyclovir) p-Hydroxybenzoic acid Download
Aciclovir syrup and injection YMC-Triart C18 Nicotinic acid Aciclovir (Acyclovir) Download
Alkaloids YMC-Triart C18 Scopolamine Atropine Cinchonine Quinine Dihydroquinine Download
Amlodipine besilate (JP 15th Edition Supplement I) YMC-Triart C18 Amlodipine besilate Isobutyl p-hydroxybenzoate (Isobutyl paraben) Download
Amlodipine besilate (JP draft|Related substances) YMC-Triart C18 Amlodipine besilate Download
Amlodipine besilate orally disintegrating tablets (JP draft|Assay) YMC-Triart C18 Amlodipine besilate Download
Antifungal agents included in a shampoo YMC-Triart C18 Hinokitiol Ciclopirox olamine Piroctone olamine (Octopirox) Download
Antimicrobial peptides YMC-Triart C18 α-Defensin-1(human) (MW 3442) α-Defensin-2(human) (MW 3371) α-Defensin-3(human) (MW 3486) Download
Aromatic carboxylic acids YMC-Triart C18 Salicylic acid Methyl p-hydroxybenzoate (Methyl paraben) trans-Cinnamic acid Download
Barbiturates in human serum YMC-Triart C18 Barbital Phenobarbital Hexobarbital Pentobarbital Secobarbital Download
Barbiturates YMC-Triart C18 Barbital Phenobarbital Hexobarbital Pentobarbital Secobarbital Download
Basic drugs YMC-Triart C18 Chlorpheniramine Dextromethorphan Propyl p-hydroxybenzoate (Propyl paraben) Download
Benzodiazepine derivatives (Triazolam, Etizolam) – LC/MS YMC-Triart C18 Triazolam Etizolam Download
Bipyridinium herbicides (diquat, paraquat) – LC/MS YMC-Triart C18 Diquat Paraquat Download
Bipyridinium herbicides (diquat, paraquat) YMC-Triart C18 Diquat Paraquat Download
Butenafine hydrochloride YMC-Triart C18 Butenafine hydrochloride Download
Carvedilol tablets (JP 16th|Assay) YMC-Triart C18 Carvedilol Isoamyl p-hydroxybenzoate (I.S.) Download
Carvedilol tablets (JP 16th|Related substances) YMC-Triart C18 Carvedilol Download
Clindamycin hydrochloride (The Japanese Pharmacopoeia 15th Edition Supplement II) YMC-Triart C18 Clindamycin hydrochloride Download
Clindamycin hydrochloride (The United States Pharmacopoeia) YMC-Triart C18 Lincomycin hydrochloride Clindamycin hydrochloride Download
Clopidogrel and related compounds YMC-Triart C18 Clopidogrel carboxylic acid Clopidogrel sulphate Clopidogrel related compound B Download
Donepezil hydrochloride (USP 37th) YMC-Triart C18 Donepezil hydrochloride Download
Donepezil hydrochloride tablets (USP 37th) YMC-Triart C18 Donepezil hydrochloride Download
Erythromycin antibiotic drugs YMC-Triart C18 Erythromycin Erythromycin ethylsuccinate Erythromycin estolate Download
Fexofenadine Hydrochloride Tablets YMC-Triart C18 Fexofenadine hydrochloride Download
Flavonoids YMC-Triart C18 Daidzein Luteolin Kaempferol Baicalein Download
Glimepiride Tablets YMC-Triart C18 Glimepiride Download
Glutathione and related compounds YMC-Triart C18 L-Cysteine L-Cystine Glutathione (GSH) Glutathione disulfide (GSSG) Download
Hinokitiol YMC-Triart C18 Hinokitiol Methyl benzoate Download
L-Isoleucine L-Leucine and L-Valine granules YMC-Triart C18 L-Valine L-Isoleucine L-Leucine Download
LC/MS analysis of oligonucleotides (siRNA) YMC-Triart C18 5′-GU ACG CGG AAU ACU UCG AdTdT-3′ (20 nt) 5′-CGU ACG CGG AAU ACU UCG AdTdT-3′ (21 nt) Download
LC/MS analysis of oligonucleotides (miRNA) YMC-Triart C18 5′-pUGG AGU GUG ACA AUG GUG UUG-3′ (21 nt) 5′-pUGG AGU GUG ACA AUG GUG UUG U-3′ (22 nt) Download
LC/MS analysis of tetracycline antibiotics YMC-Triart C18 Oxytetracycline Tetracycline Chlortetracycline Doxycycline Download
Lamivudine YMC-Triart C18 Lamivudine Download
Losartan potassium (JP 16th|Assay) YMC-Triart C18 Losartan potassium Download
Losartan potassium (JP 16th|Related substances) YMC-Triart C18 Losartan potassium Download
Losartan potassium (USP 34th| Assay) YMC-Triart C18 Losartan potassium Download
Losartan potassium (USP 34th| Chromatographic purity) YMC-Triart C18 Losartan potassium Triphenylmethanol Download
Naftopidil tablets YMC-Triart C18 Naftopidil 1-Naphthol (I.S.) Download
Nicotine and related compounds YMC-Triart C18 Anabasine hydrochloride Cotinine Nicotine Download
Nucleic acid bases YMC-Triart C18 Cytosine Uracil Guanine Thymine Adenine Download
Nucleotides YMC-Triart C18 5′-CMP 5′-UMP 5′-GMP 5′-IMP 5′-TMP 5′-AMP Download
Oligonucleotides, d(T)2-20 YMC-Triart C18 Oligodeoxythymidylic acid [d(T)2-20] Download
Organic acids YMC-Triart C18 Oxalic acid Tartaric acid Glycolic acid Formic acid L-Malic acid Malonic acid Lactic acid Acetic acid Maleic acid Citric acid Succinic acid Fumaric acid Acrylic acid Propionic acid Download
Orotic acid YMC-Triart C18 5-Fluoroorotic acid Uracil Orotic acid Download
Paeoniflorin (JP 16th|Assay) YMC-Triart C18 Albiflorin Paeoniflorin Download
Paeoniflorin in Kampo products (JP 16th|Assay) YMC-Triart C18 Albiflorin Paeoniflorin Download
Pesticides used on golf course – method transfer from HPLC to UHPLC YMC-Triart C18 Oxine-copper (Copper 8-quinolinolate) Asulam Thiram Triclopyr Mecoprop Flazasulfuron Siduron Halosulfuron-methyl Azoxystrobin Download
Pioglitazone hydrochloride tablets YMC-Triart C18 Pioglitazone hydrochloride Benzophenone Download
Pioglitazone hydrochloride YMC-Triart C18 Pioglitazone hydrochloride Benzophenone Download
Risperidone (JP 16th|Related substances) YMC-Triart C18 Risperidone Download
Risperidone Tablets and Fine Granules (JP 16th|Dissolution) YMC-Triart C18 Risperidone Download
Simultaneous separation of haloacetic acids by LC/MS YMC-Triart C18 Monochloroacetic acid Dichloroacetic acid Trichloroacetic acid Download
Simvastatin (JP 16th| Assay) YMC-Triart C18 Simvastatin Lovastatin Download
Simvastatin (USP 34th| Assay) YMC-Triart C18 Simvastatin Lovastatin Download
Simvastatin tablets (JP draft) YMC-Triart C18 Simvastatin Download
Simvastatin tablets (USP 34th| Assay) YMC-Triart C18 Simvastatin Download
Soy isoflavones in supplement YMC-Triart C18 Daidzin Glycitin Genistin 6”-O-Malonyldaidzin 6”-O-Malonylglycitin 6”-O-Acetyldaidzin 6”-O-Acetylglycitin 6”-O-Malonylgenistin Daidzein Glycitein 6”-O-Acetylgenistin Genistein (Isoflavones) Download
Soy isoflavones YMC-Triart C18 Daidzin Glycitin Genistin 6”-O-Malonyldaidzin 6”-O-Malonylglycitin 6”-O-Acetyldaidzin 6”-O-Acetylglycitin 6”-O-Malonylgenistin Daidzein Glycitein 6”-O-Acetylgenistin Genistein (Isoflavones) Download
Tacrolimus capsules YMC-Triart C18 Tacrolimus Tacrolimus 19-epimer Download
Tacrolimus hydrate (JP 16th| Assay) YMC-Triart C18 Tacrolimus hydrate Heptyl p-hydroxybenzoate (I.S.) Download
Tacrolimus YMC-Triart C18 Tacrolimus Download
Telmisartan (JP draft) YMC-Triart C18 Telmisartan Download
Valsartan, Valsartan tablets YMC-Triart C18 Valsartan Valsartan enantiomer (Valsartan related compound A) Download
Valsartan YMC-Triart C18 Valsartan Download
Water-soluble vitamins YMC-Triart C18 L-Ascorbic acid 2-glucoside L-Ascorbic acid (Vitamin C) Erythorbic acid (D-Isoascorbic acid) Nicotinic acid Nicotinamide D-(+)-Pantothenic acid calcium salt Pyridoxal hydrochloride (Vitamin B6) Pyridoxine HCl (Vitamin B6) Folic acid Thiamine HCl (Vitamin B1) Cyanocobalamin (Vitamin B12) D-Biotin (Vitamin H) Riboflavin (Vitamin B2) Download


Analytical Columns (Max. pressure : 100 MPa for 1.9 um, 45 MPa for 3 um and 5 um)

Particle size
(µm)
Pore size
(Å)
Column size
Inner diameter X length (mm)
Product Number
1.9 120 2.0 X 20 TA12SP9-0202PT
2.0 X 30 TA12SP9-0302PT
2.0 X 50 TA12SP9-0502PT
2.0 X 75 TA12SP9-L502PT
2.0 X 100 TA12SP9-1002PT
2.0 X 150 TA12SP9-1502PT
2.1 X 20 TA12SP9-02Q1PT
2.1 X 30 TA12SP9-03Q1PT
2.1 X 50 TA12SP9-05Q1PT
2.1 X 75 TA12SP9-L5Q1PT
2.1 X 100 TA12SP9-10Q1PT
2.1 X 150 TA12SP9-15Q1PT
3.0 X 50 TA12SP9-0503PT
3.0 X 75 TA12SP9-L503PT
3.0 X 100 TA12SP9-1003PT
3.0 X 150 TA12SP9-1503PT
3 120 2.1 X 20 TA12S03-02Q1PTH
2.1 X 33 TA12S03-H3Q1PTH
2.1 X 50 TA12S03-05Q1PTH
2.1 X 75 TA12S03-L5Q1PTH
2.1 X 100 TA12S03-10Q1PTH
2.1 X 150 TA12S03-15Q1PTH
3.0 X 50 TA12S03-0503PTH
3.0 X 75 TA12S03-L503PTH
3.0 X 100 TA12S03-1003PTH
3.0 X 150 TA12S03-1503PTH
4.6 X 33 TA12S03-H346PTH
4.6 X 50 TA12S03-0546PTH
4.6 X 75 TA12S03-L546PTH
4.6 X 100 TA12S03-1046PTH
4.6 X 150 TA12S03-1546PTH
4.6 X 250 TA12S03-2546PTH
5 120 2.1 X 20 TA12S05-02Q1PTH
2.1 X 33 TA12S05-H3Q1PTH
2.1 X 50 TA12S05-05Q1PTH
2.1 X 75 TA12S05-L5Q1PTH
2.1 X 100 TA12S05-10Q1PTH
2.1 X 150 TA12S05-15Q1PTH
3.0 X 50 TA12S05-0503PTH
3.0 X 75 TA12S05-L503PTH
3.0 X 100 TA12S05-1003PTH
3.0 X 150 TA12S05-1503PTH
4.0 X 150 TA12S05-1504PTH
4.0 X 250 TA12S05-2504PTH
4.6 X 33 TA12S05-H346PTH
4.6 X 50 TA12S05-0546PTH
4.6 X 75 TA12S05-L546PTH
4.6 X 100 TA12S05-1046PTH
4.6 X 150 TA12S05-1546PTH
4.6 X 250 TA12S05-2546PTH

Analytical Columns (Max. pressure : 10-25 MPa, depending on dimensions)

Particle size
(µm)
Pore size
(Å)
Column size
Inner diameter X length (mm)
Product Number
3 120 2.0 X 20 TA12S03-0202WT
2.0 X 30 TA12S03-0302WT
2.0 X 50 TA12S03-0502WT
2.0 X 75 TA12S03-L502WT
2.0 X 100 TA12S03-1002WT
2.0 X 150 TA12S03-1502WT
3.0 X 50 TA12S03-0503WT
3.0 X 75 TA12S03-L503WT
3.0 X 100 TA12S03-1003WT
3.0 X 150 TA12S03-1503WT
4.6 X 35 TA12S03-H546WT
4.6 X 50 TA12S03-0546WT
4.6 X 75 TA12S03-L546WT
4.6 X 100 TA12S03-1046WT
4.6 X 150 TA12S03-1546WT
4.6 X 250 TA12S03-2546WT
5 120 2.0 X 20 TA12S05-0202WT
2.0 X 30 TA12S05-0302WT
2.0 X 50 TA12S05-0502WT
2.0 X 75 TA12S05-L502WT
2.0 X 100 TA12S05-1002WT
2.0 X 150 TA12S05-1502WT
3.0 X 50 TA12S05-0503WT
3.0 X 75 TA12S05-L503WT
3.0 X 100 TA12S05-1003WT
3.0 X 125 TA12S05-R503WT
3.0 X 150 TA12S05-1503WT
4.0 X 125 TA12S05-R504WT
4.0 X 150 TA12S05-1504WT
4.0 X 250 TA12S05-2504WT
4.6 X 35 TA12S05-H546WT
4.6 X 50 TA12S05-0546WT
4.6 X 75 TA12S05-L546WT
4.6 X 100 TA12S05-1046WT
4.6 X 150 TA12S05-1546WT
4.6 X 250 TA12S05-2546WT
6.0 X 150 TA12S05-1506WT
6.0 X 250 TA12S05-2506WT

Semi-preparative columns

Particle size
(µm)
Pore size
(Å)
Column size
Inner diameter X length (mm)
Product Number
5 120 10 X 150 TA12S05-1510WT
10 X 250 TA12S05-2510WT

High durability semi-preparative columns (YMC-Actus Triart C18)

Particle size
(µm)
Pore size
(Å)
Column size
Inner diameter X length (mm)
Product Number
5 120 20 X 50 TA12S05-0520WX
20 X 100 TA12S05-1020WX
20 X 150 TA12S05-1520WX
20 X 250 TA12S05-2520WX
30 X 50 TA12S05-0530WX
30 X 75 TA12S05-L530WX
30 X 100 TA12S05-1030WX
30 X 150 TA12S05-1530WX
30 X 250 TA12S05-2530WX
50 X 250 TA12S05-2553AX

EXP®Guard Cartridge Columns (Max. pressure: 100 MPa, 3/pack)

Particle size
(µm)
Pore size
(Å)
Column size
Inner diameter X length (mm)
Product Number
1.9 120 2.1 X 5 TA12SP9-E5Q1CC
3.0 X 5 TA12SP9-E503CC

*When using it for the first time, please purchase the EXP®Guard Cartridge Holder.
*EXP is a registered trademark of Optimize Technologies, Inc.

Guard Cartridge Columns

Particle size
(µm)
Pore size
(Å)
Column size
Inner diameter X length (mm)
/pack Product Number
3 120 2.1 X 10 5 TA12S03-01Q1GC
1 TA12S03-01Q1GC-1
2.1 X 20 5 TA12S03-02Q1GC
1 TA12S03-02Q1GC-1
3.0 X 10 5 TA12S03-0103GC
1 TA12S03-0103GC-1
3.0 X 20 5 TA12S03-0203GC
1 TA12S03-0203GC-1
4.0 X 10 5 TA12S03-0104GC
1 TA12S03-0104GC-1
4.0 X 20 5 TA12S03-0204GC
1 TA12S03-0204GC-1
5 120 2.1 X 10 5 TA12S05-01Q1GC
1 TA12S05-01Q1GC-1
2.1 X 20 5 TA12S05-02Q1GC
1 TA12S05-02Q1GC-1
3.0 X 10 5 TA12S05-0103GC
1 TA12S05-0103GC-1
3.0 X 20 5 TA12S05-0203GC
1 TA12S05-0203GC-1
4.0 X 10 5 TA12S05-0104GC
1 TA12S05-0104GC-1
4.0 X 20 5 TA12S05-0204GC
1 TA12S05-0204GC-1
10 X 10 2 TA12S05-0110CC
20 X 10 2 TA12S05-0120CCN
30 X 10 2 TA12S05-0130CCN

*When using it for the first time, please purchase the cartridge holder corresponding to size of column.

Cartridge Holder

Product Number
EXP®direct connect holder (Adjustable ferrule, Pressure limit: 137 MPa)
(2 titanium hybrid ferrules and 1 nut included)
XPCHUHP
Cartridge holder length: 10 mm XPGCHP1
length: 20 mm XPGCHP2
Semi-prep Cartridge Holder Inner diameter: 10 mm XPCHSPW1
Inner diameter: 20 mm XPGHFSP20ID
Inner diameter: 30 mm XPGHFSP30ID

*EXP is a registered trademark of Optimize Technologies, Inc.