USP No | Functional group | USP Description | YMC Product |
---|---|---|---|
L01 | C18 | Octadecyl silane <ODS or C18> chemically bonded to porous silica or ceramic particles – 1.5 to 10 µm in diameter | YMC-Triart C18 |
L03 | SIL | Porous silica particles – 1.5 to 10 µm in diameter | |
L07 | C8 | Octyl silane <C8> chemically bonded to porous silica particles – 1.5 to 10 µm in diameter | YMC-Triart C8 |
L08 | NH2 | An essentially monomolecular layer of aminopropylsilane <NH2> chemically bonded to totally porous silica gel support – 1.5 to 10 µm in diameter | YMC-Pack NH2 |
L10 | CN | Nitrile groups <CN> chemically bonded to porous silica particles – 1.5 to 10 µm in diameter | YMC-Pack CN |
L11 | Ph | Phenyl groups chemically bonded to porous silica particles – 1.5 to 10 µm in diameter | YMC-Triart Phenyl |
L13 | C1 | Trimethylsilane <C1> chemically bonded to porous silica particles – 1.5 to 10 µm in diameter | YMC-Pack TMS |
L20 | Diol | Dihydroxypropane groups chemically bonded to porous silica particles – 1.5 to 10 µm in diameter | YMC-Triart Diol-HILIC |
L24 | Polyvinyl alcohol | Polyvinyl alcohol chemically bonded to porous silica particles – 5 µm in diameter | YMC-Pack PVA-Sil |
L26 | C4 | Butyl silane <C4> chemically bonded to porous silica particles – 1.5 to 10 µm in diameter | YMC-Triart Bio C4 |
L27 | SIL | Porous silica particles – 30 to 50 µm in diameter | YMC-Pack SIL-HG |
L33 | Diol | Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability | YMC-Pack Diol (SEC) |
L40 | Cellulose derivatives |
Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 3 to 20 µm in diameter | CHIRAL ART Cellulose-C |
L43 | PFP | Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 µm in diameter | YMC-Triart PFP |
L51 | Amylose derivatives |
Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 3 to 20 µm in diameter | CHIRAL ART Amylose-C |
L59 | Diol | Packing for the size exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. It is spherical (1.5 to 10 µm), silica or hybrid packing with a hydrophilic coating | YMC-SEC-MAB |
L62 | C30 | C30 silane bonded phase on a fully porous spherical silica, 3 to 15 µm in diameter | YMC Carotenoid |
L99 | Amylose derivatives |
Amylose tris-(3,5-dimethylphenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter | CHIRAL ART Amylose-SA |
L111 | Polyamine | Polyamine chemically bonded to porous spherical silica particles, 5 µm in diameter | YMC-Pack Polyamine II |
L119 | Cellulose derivatives |
Cellulose tris-(3,5-dichlorophenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter. | CHIRAL ART Cellulose-SC |
Exhibitaion 展覽資訊
AMERICAS
USA
Show name | Dates | Location |
---|---|---|
TIDES | 20 May. – 23 May., 2019 | San Diego, CA |
PREP 2019 | 7 Jul. -10 Jul., 2019 | Baltimore, MD |
SFC 2019 | 29 Sep. -1 Oct., 2019 | Philadelphia, PA |
2019 EAS | 18 Nov. -20 Nov., 2019 | Princeton, NJ |
PITTCON 2020 | 1 Mar. – 5 Mar., 2020 | Chicago, IL |
EUROPE
Belgium
Show name | Dates | Location |
---|---|---|
Laborama 2019 | 14 Mar. – 15 Mar., 2019 | Brussels |
Austria
Show name | Dates | Location |
---|---|---|
LAB Supply | 19 Mar., 2019 | Vienna |
Germany
Show name | Dates | Location |
---|---|---|
LAB Supply | 3 Apr., 2019 | Frankfurt-Hoechst |
LAB Supply | 15 May., 2019 | Leverkusen |
LAB Supply | 13 Jun., 2019 | Berlin |
LAB Supply | 28 Aug., 2019 | Dresden |
BioTalk Summit | 23 Sep. – 24 Sep., 2019 | Berlin |
LAB Supply | 25 Sep., 2019 | Münster |
LAB Supply | 29 Oct., 2019 | Hamburg |
4. IUTA AnalytikTag | 07 Nov., 2019 | Duisburg |
Italy
Show name | Dates | Location |
---|---|---|
HPLC 2019 | 16 Jun. – 20 Jun., 2019 | Milan |
ASIA
Korea
Show name | Dates | Location |
---|---|---|
The Pharmaceutical Society of Korea | 29 Jan., 2019 | Daegu |
2019 KOREA LAB | 16 Apr. – 19 Apr., 2019 | Goyang |
The Korean Society for Applied Biological Chemistry | 20 Jun. – 22 Jun., 2019 | Busan |
Korean Chemical Society | 16 Oct. – 18 Oct., 2019 | Changwon |
The Korean Society of Pharmacognosy | 28 Nov., 2019 | Seoul |
India
Show name | Dates | Location |
---|---|---|
INDIA LAB EXPO | 19 Sep. – 21 Sep., 2019 | Hyderabad |
CPhI india P-mec | 26 Nov. – 28 Nov., 2019 | Greater Noida |
WEBINAR SERIES
mAbs / Peptide / Oligo / ADC Purification & Production Learning Series
分離/純化方法開發策略-Strategy of method development for isolation/purification
生產級(Pilot or production scale)/製備型液相層析(Preparative HPLC /MPLC)與實驗室液相層析(HPLC)的分離純化(purification)原理和方法相同,但由於進樣量與層析管柱尺寸大小差異,製備級純化需考慮生產成本並有效收集目標成分(並達到要求規格,例如純度),因此分離純化策略上需與實驗室採取不同的角度來選擇分離條件。
製備級純化方法開發步驟 Steps for preparative purification
1. 分析級條件確認 Method development on the analytical scale
■ 選擇層析分離模式(膠體填料) Separation mode (packing material)
■ 分析條件確認 Separation method
2. 樣品進樣量確認 Loading study
■ 進樣濃度與體積 Concentration Load and Volume Load
■ 進樣量最佳化 Loading amount
■ 檢測條件最佳化 Detection
3. 製程純化放大 Consideration for scaling up
■ 純化放大規模選擇 Selection guide for the preparative scale
■ 管柱純化膠體之粒徑選擇 Selection of the particle size
4. 放大製備純化條件最佳化 Method optimization on the preparative scale
■ 分離條件最佳化 Separation method optimization
■ 選擇合適之分離純化收集系統 Preparative system evaluation
5. 進行樣品純化 Performing preparative separation
製備純化放大之方法說明歡迎來信索取
Optimization of oligonucleotide separations on ion-exchange chromatography
Nucleic acid therapeutics such as antisense, siRNA and aptamers are expected to play an important role as next-generation pharmaceuticals together with antibody drugs. These drugs demand chromatographic purification and analysis that can recognize slight structural differences following synthesis.
On this page, we provide useful tips for optimization of ion-exchange chromatography methods for oligonucleotides.
Read more “Optimization of oligonucleotide separations on ion-exchange chromatography”
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