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YMC Taiwan Co., Ltd.Column

YMC Classics HPLC Columns 液相層析管柱

產品

HPLC columns 液相層析管柱

In order to develop a successful HPLC method, the choice of the optimal selectivity is essential to establish efficient separation conditions. The best suited packing material depends significantly on the characteristics of the separation conditions, which should be thoroughly considered.

For this purpose YMC offers a wide variety of selectivities applicable to HPLC from nano-scale analysis to large scale isolation. In addition to the best-selling YMC-Pack ODS series YMC has several other phases in its portfolio to fulfil customer’s separation requirements.

YMC’s best-selling, world-renowned YMC-Pack ODS-series is widely known for its variety of selectivities for nonpolar, moderately polar and polar compounds. Further, YMC-Pack ODS-AQ was the first so called “AQ-column”, which can be used under 100% aqueous conditions! Due to their high quality and reproducibility, YMC-Pack ODS columns are successfully used for the validation of analytical HPLC methods and for long term reproducible preparative HPLC processes.

YMC-Pack (Reversed-Phase)

USP
Class		 Pore size
(Å)		 Particle size
(µm)		 Carbon Contents
(%)		 Silanol treatment Usable pH range
YMC-Pack ODS-A L1 120 3, 5, 10 17 Yes 2-7.5
200 5 12
300 3, 5 7
YMC-Pack ODS-AM 120 3, 5 17
YMC-Pack ODS-AQ 120 3, 5 14
200 5 10
YMC-Pack ODS-AL 120 5 17 No
YMC-Pack PolymerC18 – – 6 – – 2-13
YMC-Pack C8 L7 120 3, 5, 10 10 Yes 2-7.5
200 5 7
300 5 4
YMC-Pack C4 L26 120 3, 5, 10 7
200 5 5
300 5 3
YMC-Pack TMS L13 120 3, 5 4
YMC-Pack Ph L11 120 3, 5 9
YMC-Pack CN L10 120 3, 5 7
300 5 3
YMC-Pack PROTEIN-RP L26 200 5 4 – 1.5-7.5
YMCbasic L7 200 3, 5 7 Yes 2-7.5
YMC Carotenoid L62 – 3, 5 – – 2-7.5

YMC-Pack (Normal-Phase/HILIC)

USP
Class		 Pore size
(Å)		 Particle size
(µm)		 Usable pH range
YMC-Pack SIL L3 120 3, 5, 10 2-7.5
YMC-Pack SIL-06 60 5
YMC-Pack CN L10 120 5
YMC-Pack Diol-NP L20 60 5
120 5
YMC-Pack Polyamine II L111 120 5
YMC-Pack NH2 L8 120 5
YMC-Pack PA-G – 120 5 4-7.5
YMC-Pack PVA-Sil L24 120 5 2-9.5

J’sphere

USP
Class		 Pore size
(Å)		 Particle size
(µm)		 Carbon Contents
(%)		 Silanol treatment Usable pH range
J’sphere ODS-H80 L1 80 4 22 Yes 1-9
J’sphere ODS-M80 80 4 14 2-7.5
J’sphere ODS-L80 80 4 9

YMC-Pack ProFamily

產品

YMC ProFamily is based on a silica with an extremely low metal content. Low metal content suppresses polar interactions, as metal impurities cause silanol groups to become more acidic. This provides YMC ProFamily excellent resolution for a wide range of compounds, especially basic pharmaceuticals.

To minimise the unwanted interactions between residual silanols and the sample molecules that are responsible for asymmetric peaks, YMC utilises a proprietary, highly effective endcapping process.

Important for every chromatographer: YMC ensures that the physical properties e.g. particle size, pore size, metal content are extremely reproducible from LOT to LOT.

USP
Class		 Pore size
(Å)		 Particle size
(µm)		 Carbon Contents
(%)		 Silanol treatment Usable pH range
YMC-UltraHT Pro C18 L1 120 2 16 Yes 2-8
YMC-Pack Pro C18 3, 5,10
YMC-UltraHT Hydrosphere C18 2 12
Hydrosphere C18 3, 5
YMC-Pack Pro C18 RS 80 3, 5 22 2-10
YMC-Pack Pro C8 L7 120 3, 5 10 2-7.5
YMC-Pack Pro C4 L26 120 3, 5 7

YMC-Triart Series

產品

YMC-Triart Series are organic/inorganic hybrid silica based columns that emphasize versatility. The main features are superior durability, good peak shape for all kinds of compounds, and reproducibility. With the same selectivity across different particle sizes, smooth method transfer between UHPLC and HPLC can be performed.
Moreover, alternative bonded phases such as C8 and HILIC supplement the performance of C18, allowing separations that C18 cannot achieve. For separation of proteins including antibodies, YMC-Triart Bio C4 with wide pore is effective.
YMC-Triart Prep, preparative packing materials with great chemical stability are also available.

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Columns

Separation
mode		 USP
Class No.		 Particle size
(μm)		 Pore size
(Å)		 Carbon content
(%)*		 Usable
pH range		 100% aqueous
compatibility
Triart C18 Reversed-phase L1 1.9
3
5		 120 20 1 – 12 ○
Triart C18 ExRS L1 80 25 ×
Triart C8 L7 120 17 ×
Triart Phenyl L11 17 1 – 10 ○
Triart PFP L43 15 1 – 8 ○
Triart Bio C18 L1 300 – 1 – 12 ○
Triart Bio C4 L26 – 1 – 10 ○
Triart Diol-HILIC HILIC L20 120 – 2 – 10 –
Triart SIL Normal-phase
HILIC		 L3 5 120 – 2 – 8 –

*Containing carbon content for hybrid silica base material.

>相關(半)製備級管柱介紹 YMC-Actus

>無金屬接觸管柱介紹 Metal Free Column

Packing materials

Product Particle size
(µm)		 Pore size
(Å)		 Carbon content
(%)		 Usable pH range
Triart SIL 3, 5 120 – –
Triart C18 20 1-12
Triart C8 17
Triart Prep SIL 7, 10, 15, 20 120 – –
Triart Prep C18-S 20 2-10
Triart Prep C8-S 10, 15, 20 17
Triart Prep Bio200 C8 10 200 14

特點

-Great chemical durability in an expanded pH range provided by hybrid particles
-Great peak shapes without adsorption/peak tailing
-Identical selectivity and excellent peak shapes across various particle sizes
-Comparison of separation selectivity among YMC-Triart columns

-High throughput by shortening analysis time under high flow rate condition

With innovative surface modification on organic hybrid silica, YMC-Triart columns show great chemical durability and can be used over a wide pH range. Even at high-pH or high-temperature conditions, the lifetimes of YMC-Triart C18 and C8 are more than 10 times greater than those of conventional C18 columns and a few times greater than those of commercially available high-alkaline-resistant C18 columns.

Durability at a high pH. pH 11.5, 40ºC

Column 5 µm, 150 X 4.6 mmI.D.
Eluent 50 mM K2HPO4-K3PO4 (pH 11.5)/methanol (90/10)
Flow rate 1.0 mL/min
Temperature 40ºC
Sample benzyl alcohol

Durability at a low pH at a high temperature. pH 1, 70ºC

Test condition

The columns are kept in acetonitrile/water/TFA (10/90/1, pH 1) at 70ºC, and tested for performance every 20 hours under the condition of column inspection below.

Column inspection
Column 5 µm, 50 X 2.0 mmI.D.
Eluent acetonitrile/water (60/40)
Flow rate 0.2 mL/min
Temperature 37ºC
Sample butyl benzoate

-Great peak shapes without adsorption/peak tailing

The peak tailing or fronting of ionic compounds is often caused by adsorption to residual silanol groups and/or surface impurities resulting from the base material or manufacturing processes.
YMC-Triart, based on a hybrid silica material with low metal impurities and rigorously endcapped, provides symmetrical peak shapes for all types of compounds.

Basic compounds

Eluent 20 mM KH2PO4-K2HPO4 (pH 6.9) /acetonitrile (65/35)
Detection UV at 235 nm
Sample 1. Chlorpheniramine
2. Dextromethorphan
3. Propyl paraben (I.S.)


Coordination compounds

Eluent acetonitrile/0.1% H3PO4 (40/60)
Detection UV at 254 nm
Sample 1. Hinokitiol
2. Methyl benzoate (I.S.)

Acidic compounds

Eluent 10 mM CH3COOH-CH3COONH4 (pH 4.2) /acetonitrile (75/25)
Detection UV at 254 nm
Sample 1. Salicylic acid
2. Methyl paraben (I.S.)
3. Cinnamic acid

 

Column 150 X 3.0 mmI.D. or 150 X 4.6 mmI.D.
Flow rate 0.425 mL/min for 3.0 mmI.D.,
1.0 mL/min for 4.6 mmI.D.
Temperature 40ºC

-Identical selectivity and excellent peak shapes across various particle sizes

YMC-Triart columns have identical selectivity and excellent peak shapes for basic (ionic) compounds across all particle sizes, including 1.9 μm. This allows predictable scale-up from UHPLC to conventional HPLC and even to semi-preparative LC, and vice versa. In contrast, commercially available C18 columns often show some differences in selectivity, retention, and peak shape between different particle sizes.

Basic compounds

1.Chlorpheniramine
2.Dextromethorphan
3.Propyl paraben(I.S.)

Column 50 X 2.0 mmI.D. or 2.1 mmI.D.
Eluent 20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)
Flow rate 0.2 mL/min
Temperature 40℃
Detection UV at 235 nm

-Comparison of separation selectivity among YMC-Triart columns

A mixture that consists of compounds with various characteristics is analyzed with reversed-phase Triart columns. In addition to hydrophobic interactions, secondary interactions such as π–π interactions and polar interactions differ from column to column. These parameters have a great impact on the retention capacity (k′) and separation factor (α). By utilizing the differences in the separation characteristics, a wide range of compounds can be well separated with Triart columns.

1.Amitriptyline
2.8-Quinolinol
3.Testosterone
4.Naphthalene
5.Ibuprofen
6.Propylbenzene
7.n-Butylbenzene
8.o-Terphenyl
9.Triphenylene

Column 5 µm, 150 X 3.0 mmI.D.
Eluent 20 mM KH2PO4-H3PO4 (pH 3.1)/methanol (25/75)
Flow rate 0.425 mL/min
Temperature 40℃
Detection UV at 265 nm
Injection 4 µL

UHPLC

YMC-Triart series 1.9 µm columns based on novel, multi-layered, organic/inorganic hybrid silica particles are designed for UHPLC. The outstanding mechanical strength of Triart series 1.9 µm makes it ideal for use at higher pressures typical of UHPLC.

–Tips for method transfer among different particle sizes

-Suitable for ultra-fast separation
-Useful for high resolution analysis
-Excellent resolution without adsorption and tailing

-Suitable for ultra-fast separation

YMC-Triart series 1.9 µm offers high resolution with short length column. Since its efficiency does not decrease even if the flow rate increases, it enables ultra high throughput analysis.

Ultra-fast separation of drugs

1. Hydrochlorothiazide
2. Valsartan
3. Losartan potassium
4. Amlodipine besilate
5. Atorvastatin calcium hydrate
6. Candesartan cilexetil

Eluent A) 10 mM CH3COONH4-CH3COOH (pH 5.5)
B) acetonitrile
Detection UV at 254 nm
Temperature 30℃
Injection 2 µL

-Useful for high resolution analysis

YMC-Triart series 1.9 µm has superior column efficiency. It is suitable for multi-component separation such as peptide mapping that requires high resolution.

Peptide mapping

Column YMC-Triart C18
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.08)
5-40%B (0-15 min) for a single column
5-40%B (0-30 min) for two coupled columns
Flow rate 0.4 mL/min
Temperature 70℃
Detection UV at 220 nm
Injection 10 µL for a single column
20 µL for two coupled columns
Sample Tryptic digest of Bovine Hemoglobin
System Agilent 1290

-Excellent resolution without adsorption and tailing

YMC-Triart series 1.9 µm’s innovative surface modification technology results in excellent peak shapes even for the basic and coordination compounds that often exhibit adsorption or tailing shapes on other UHPLC columns. Thus, its quantitative capability is sufficient, and decreases the risk of missing unknown constituents. YMC-Triart series stands head and shoulders above any other columns in quantitative capability.

Comparison of chromatographic performance for ionic compounds with UHPLC column

Basic compounds
Column 50 X 2.0 mmI.D. or 50 X 2.1 mmI.D.
Eluent 20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)
Flow rate 0.2 mL/min
Temperature 40℃
Detection UV at 235 nm
Sample 1. Chlorpheniramine
2. Dextromethorphan
3. Propyl paraben (I.S.)
Coordination compound
Column 50 X 2.0 mmI.D. or 50 X 2.1 mmI.D.
Eluent acetonitrile/0.1% H3PO4 (40/60)
Flow rate 0.2 mL/min
Temperature 40℃
Detection UV at 254 nm
Sample 1. Hinokitiol
2. Methyl benzoate (I.S.)
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