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YMC-Actus SP columns are prepacked large-scale preparative HPLC columns. Since columns packed by YMC are QC tested prior to shipping, they arrive at your lab ready for use. Their space-saving and wheeled design allows for maximum flexibility even with limited lab space.
YMC-Actus SP columns are ideal for highly potent, toxic, or radioactive projects. Hazardous compounds often require dedicated systems and hardware, rendering traditional DAC columns impractical. Prepacked YMC-Actus SP columns eliminate the need to spend time and resources ensuring that a column has been sufficiently cleaned, packed, and validated for re-use.
| Inner diameter (mm) | Dimension (WxDxH mm) |
Weight (kg) | Connections |
|---|---|---|---|
| 80 | 480×480×990 | 60 | 1/8″ female nut |
| 100 | 480×480×1080 | 80 | 1/8″ female nut |
| 150 | 480×480×1130 | 160 | 1/8″ female nut |
| 200 | 560×560×1350 | 200 | 1/4″ female nut |
| 300 | 650×650×1350 | 540 | 1/4″ female nut |
YMC-Actus SP columns have a smaller footprint than Dynamic Axial Compression columns (DAD) because of the absence of a cylinder on the top. They can be installed in a small space and are easy to move with a cart with casters. They are also suitable for the Contichrom TWIN continuous chromatography system, which requires two columns.
Since they are pre-packed columns, no packing work by a worker is required. They can be used immediately after delivery, which would shorten manufacturing downtime. YMC-Actus SP columns will be provided with individual inspection reports.
YMC-Actus SP columns use cylinders to pack the packing material by physical compression. The pistons are fixed in place after compression. After packing, the packed beds are kept compressed by springs in the pistons. Therefore, they provide higher pressure resistance and durability.
A conventional column decreased its performance just by applying 2 MPa back pressure. On the other hand, the YMC-Actus SP column maintained its initial theoretical plate and tailing factor after applying 7 MPa.
| Column | YMC-Triart Prep C18-S (10 µm, 120 Å) 250 × 100 mm I.D. |
|---|---|
| Eluent | methanol/water (85/15) |
| Flow rate | 200 mL/min |
| Sample | 1. methylbenzoate 2. toluene |
| Inner diameter (mm) | Bed length (mm) |
|---|---|
| 80, 100, 150, 200, 300 | 150, 250 |
| Packing material | Particle size (µm) | Pore size (Å) | |
|---|---|---|---|
| YMC-Triart Prep | C18-S | 7 | 120 |
| 10 | |||
| 15 | |||
| 20 | |||
| C8-S | 10 | 120 | |
| 15 | |||
| 20 | |||
| Bio200 C8 | 10 | 200 | |
| C4-S | 10 | 120 | |
| Phenyl-S | 10 | 120 | |
| YMC🞶GEL | ODS-A-HG | 7 | 120 |
| 10 | |||
| 15 | |||
| 20 | |||
| ODS-AQ-HG | 7 | 120 | |
| 10 | |||
| 15 | |||
| 20 | |||
*The wheeled stand is standard equipment.
*Inquiry us for other packing material and column size.
In order to develop a successful HPLC method, the choice of the optimal selectivity is essential to establish efficient separation conditions. The best suited packing material depends significantly on the characteristics of the separation conditions, which should be thoroughly considered.
For this purpose YMC offers a wide variety of selectivities applicable to HPLC from nano-scale analysis to large scale isolation. In addition to the best-selling YMC-Pack ODS series YMC has several other phases in its portfolio to fulfil customer’s separation requirements.
YMC’s best-selling, world-renowned YMC-Pack ODS-series is widely known for its variety of selectivities for nonpolar, moderately polar and polar compounds. Further, YMC-Pack ODS-AQ was the first so called “AQ-column”, which can be used under 100% aqueous conditions! Due to their high quality and reproducibility, YMC-Pack ODS columns are successfully used for the validation of analytical HPLC methods and for long term reproducible preparative HPLC processes.
| USP Class |
Pore size (Å) |
Particle size (µm) |
Carbon Contents (%) |
Silanol treatment | Usable pH range | |
|---|---|---|---|---|---|---|
| YMC-Pack ODS-A | L1 | 120 | 3, 5, 10 | 17 | Yes | 2-7.5 |
| 200 | 5 | 12 | ||||
| 300 | 3, 5 | 7 | ||||
| YMC-Pack ODS-AM | 120 | 3, 5 | 17 | |||
| YMC-Pack ODS-AQ | 120 | 3, 5 | 14 | |||
| 200 | 5 | 10 | ||||
| YMC-Pack ODS-AL | 120 | 5 | 17 | No | ||
| YMC-Pack PolymerC18 | – | – | 6 | – | – | 2-13 |
| YMC-Pack C8 | L7 | 120 | 3, 5, 10 | 10 | Yes | 2-7.5 |
| 200 | 5 | 7 | ||||
| 300 | 5 | 4 | ||||
| YMC-Pack C4 | L26 | 120 | 3, 5, 10 | 7 | ||
| 200 | 5 | 5 | ||||
| 300 | 5 | 3 | ||||
| YMC-Pack TMS | L13 | 120 | 3, 5 | 4 | ||
| YMC-Pack Ph | L11 | 120 | 3, 5 | 9 | ||
| YMC-Pack CN | L10 | 120 | 3, 5 | 7 | ||
| 300 | 5 | 3 | ||||
| YMC-Pack PROTEIN-RP | L26 | 200 | 5 | 4 | – | 1.5-7.5 |
| YMCbasic | L7 | 200 | 3, 5 | 7 | Yes | 2-7.5 |
| YMC Carotenoid | L62 | – | 3, 5 | – | – | 2-7.5 |
| USP Class |
Pore size (Å) |
Particle size (µm) |
Usable pH range | |
|---|---|---|---|---|
| YMC-Pack SIL | L3 | 120 | 3, 5, 10 | 2-7.5 |
| YMC-Pack SIL-06 | 60 | 5 | ||
| YMC-Pack CN | L10 | 120 | 5 | |
| YMC-Pack Diol-NP | L20 | 60 | 5 | |
| 120 | 5 | |||
| YMC-Pack Polyamine II | L111 | 120 | 5 | |
| YMC-Pack NH2 | L8 | 120 | 5 | |
| YMC-Pack PA-G | – | 120 | 5 | 4-7.5 |
| YMC-Pack PVA-Sil | L24 | 120 | 5 | 2-9.5 |
| USP Class |
Pore size (Å) |
Particle size (µm) |
Carbon Contents (%) |
Silanol treatment | Usable pH range | |
|---|---|---|---|---|---|---|
| J’sphere ODS-H80 | L1 | 80 | 4 | 22 | Yes | 1-9 |
| J’sphere ODS-M80 | 80 | 4 | 14 | 2-7.5 | ||
| J’sphere ODS-L80 | 80 | 4 | 9 |
YMC ProFamily is based on a silica with an extremely low metal content. Low metal content suppresses polar interactions, as metal impurities cause silanol groups to become more acidic. This provides YMC ProFamily excellent resolution for a wide range of compounds, especially basic pharmaceuticals.
To minimise the unwanted interactions between residual silanols and the sample molecules that are responsible for asymmetric peaks, YMC utilises a proprietary, highly effective endcapping process.
Important for every chromatographer: YMC ensures that the physical properties e.g. particle size, pore size, metal content are extremely reproducible from LOT to LOT.
| USP Class |
Pore size (Å) |
Particle size (µm) |
Carbon Contents (%) |
Silanol treatment | Usable pH range | |
|---|---|---|---|---|---|---|
| YMC-UltraHT Pro C18 | L1 | 120 | 2 | 16 | Yes | 2-8 |
| YMC-Pack Pro C18 | 3, 5,10 | |||||
| YMC-UltraHT Hydrosphere C18 | 2 | 12 | ||||
| Hydrosphere C18 | 3, 5 | |||||
| YMC-Pack Pro C18 RS | 80 | 3, 5 | 22 | 2-10 | ||
| YMC-Pack Pro C8 | L7 | 120 | 3, 5 | 10 | 2-7.5 | |
| YMC-Pack Pro C4 | L26 | 120 | 3, 5 | 7 |
YMC-Triart Series are organic/inorganic hybrid silica based columns that emphasize versatility. The main features are superior durability, good peak shape for all kinds of compounds, and reproducibility. With the same selectivity across different particle sizes, smooth method transfer between UHPLC and HPLC can be performed.
Moreover, alternative bonded phases such as C8 and HILIC supplement the performance of C18, allowing separations that C18 cannot achieve. For separation of proteins including antibodies, YMC-Triart Bio C4 with wide pore is effective.
YMC-Triart Prep, preparative packing materials with great chemical stability are also available.
| Product Name | Separation mode |
USP Class No. |
Particle size (μm) |
Pore size (Å) |
Carbon content (%)* |
Usable pH range |
100% aqueous compatibility |
|---|---|---|---|---|---|---|---|
| Triart C18 | Reversed-phase | L1 | 1.9 3 5 |
120 | 20 | 1 – 12 | ○ |
| Triart C18 ExRS | L1 | 80 | 25 | × | |||
| Triart C8 | L7 | 120 | 17 | × | |||
| Triart Phenyl | L11 | 17 | 1 – 10 | ○ | |||
| Triart PFP | L43 | 15 | 1 – 8 | ○ | |||
| Triart Bio C18 | L1 | 300 | – | 1 – 12 | ○ | ||
| Triart Bio C4 | L26 | – | 1 – 10 | ○ | |||
| Triart Diol-HILIC | HILIC | L20 | 120 | – | 2 – 10 | – | |
| Triart SIL | Normal-phase HILIC |
L3 | 5 | 120 | – | 2 – 8 | – |
| Product | Particle size (µm) |
Pore size (Å) |
Carbon content (%) |
Usable pH range |
|---|---|---|---|---|
| Triart SIL | 3, 5 | 120 | – | – |
| Triart C18 | 20 | 1-12 | ||
| Triart C8 | 17 | |||
| Triart Prep SIL | 7, 10, 15, 20 | 120 | – | – |
| Triart Prep C18-S | 20 | 2-10 | ||
| Triart Prep C8-S | 10, 15, 20 | 17 | ||
| Triart Prep Bio200 C8 | 10 | 200 | 14 |
With innovative surface modification on organic hybrid silica, YMC-Triart columns show great chemical durability and can be used over a wide pH range. Even at high-pH or high-temperature conditions, the lifetimes of YMC-Triart C18 and C8 are more than 10 times greater than those of conventional C18 columns and a few times greater than those of commercially available high-alkaline-resistant C18 columns.
Durability at a high pH. pH 11.5, 40ºC
| Column | 5 µm, 150 X 4.6 mmI.D. |
|---|---|
| Eluent | 50 mM K2HPO4-K3PO4 (pH 11.5)/methanol (90/10) |
| Flow rate | 1.0 mL/min |
| Temperature | 40ºC |
| Sample | benzyl alcohol |
Durability at a low pH at a high temperature. pH 1, 70ºC
Test condition
The columns are kept in acetonitrile/water/TFA (10/90/1, pH 1) at 70ºC, and tested for performance every 20 hours under the condition of column inspection below.
| Column inspection | |
|---|---|
| Column | 5 µm, 50 X 2.0 mmI.D. |
| Eluent | acetonitrile/water (60/40) |
| Flow rate | 0.2 mL/min |
| Temperature | 37ºC |
| Sample | butyl benzoate |
The peak tailing or fronting of ionic compounds is often caused by adsorption to residual silanol groups and/or surface impurities resulting from the base material or manufacturing processes.
YMC-Triart, based on a hybrid silica material with low metal impurities and rigorously endcapped, provides symmetrical peak shapes for all types of compounds.
Basic compounds
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9) /acetonitrile (65/35) |
|---|---|
| Detection | UV at 235 nm |
| Sample | 1. Chlorpheniramine 2. Dextromethorphan 3. Propyl paraben (I.S.) |
Coordination compounds
| Eluent | acetonitrile/0.1% H3PO4 (40/60) |
|---|---|
| Detection | UV at 254 nm |
| Sample | 1. Hinokitiol 2. Methyl benzoate (I.S.) |
Acidic compounds
| Eluent | 10 mM CH3COOH-CH3COONH4 (pH 4.2) /acetonitrile (75/25) |
|---|---|
| Detection | UV at 254 nm |
| Sample | 1. Salicylic acid 2. Methyl paraben (I.S.) 3. Cinnamic acid |
| Column | 150 X 3.0 mmI.D. or 150 X 4.6 mmI.D. |
|---|---|
| Flow rate | 0.425 mL/min for 3.0 mmI.D., 1.0 mL/min for 4.6 mmI.D. |
| Temperature | 40ºC |
YMC-Triart columns have identical selectivity and excellent peak shapes for basic (ionic) compounds across all particle sizes, including 1.9 μm. This allows predictable scale-up from UHPLC to conventional HPLC and even to semi-preparative LC, and vice versa. In contrast, commercially available C18 columns often show some differences in selectivity, retention, and peak shape between different particle sizes.
Basic compounds
1.Chlorpheniramine
2.Dextromethorphan
3.Propyl paraben(I.S.)
| Column | 50 X 2.0 mmI.D. or 2.1 mmI.D. |
|---|---|
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35) |
| Flow rate | 0.2 mL/min |
| Temperature | 40ºC |
| Detection | UV at 235 nm |
A mixture that consists of compounds with various characteristics is analyzed with reversed-phase Triart columns. In addition to hydrophobic interactions, secondary interactions such as π–π interactions and polar interactions differ from column to column. These parameters have a great impact on the retention capacity (k′) and separation factor (α). By utilizing the differences in the separation characteristics, a wide range of compounds can be well separated with Triart columns.
1.Amitriptyline
2.8-Quinolinol
3.Testosterone
4.Naphthalene
5.Ibuprofen
6.Propylbenzene
7.n-Butylbenzene
8.o-Terphenyl
9.Triphenylene
| Column | 5 µm, 150 X 3.0 mmI.D. |
|---|---|
| Eluent | 20 mM KH2PO4-H3PO4 (pH 3.1)/methanol (25/75) |
| Flow rate | 0.425 mL/min |
| Temperature | 40ºC |
| Detection | UV at 265 nm |
| Injection | 4 µL |
YMC-Triart series 1.9 µm columns based on novel, multi-layered, organic/inorganic hybrid silica particles are designed for UHPLC. The outstanding mechanical strength of Triart series 1.9 µm makes it ideal for use at higher pressures typical of UHPLC.
YMC-Triart series 1.9 µm offers high resolution with short length column. Since its efficiency does not decrease even if the flow rate increases, it enables ultra high throughput analysis.
Ultra-fast separation of drugs
1. Hydrochlorothiazide
2. Valsartan
3. Losartan potassium
4. Amlodipine besilate
5. Atorvastatin calcium hydrate
6. Candesartan cilexetil
| Eluent | A) 10 mM CH3COONH4-CH3COOH (pH 5.5) B) acetonitrile |
|---|---|
| Detection | UV at 254 nm |
| Temperature | 30℃ |
| Injection | 2 µL |
YMC-Triart series 1.9 µm has superior column efficiency. It is suitable for multi-component separation such as peptide mapping that requires high resolution.
Peptide mapping
| Column | YMC-Triart C18 |
|---|---|
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.08) 5-40%B (0-15 min) for a single column 5-40%B (0-30 min) for two coupled columns |
| Flow rate | 0.4 mL/min |
| Temperature | 70℃ |
| Detection | UV at 220 nm |
| Injection | 10 µL for a single column 20 µL for two coupled columns |
| Sample | Tryptic digest of Bovine Hemoglobin |
| System | Agilent 1290 |
YMC-Triart series 1.9 µm’s innovative surface modification technology results in excellent peak shapes even for the basic and coordination compounds that often exhibit adsorption or tailing shapes on other UHPLC columns. Thus, its quantitative capability is sufficient, and decreases the risk of missing unknown constituents. YMC-Triart series stands head and shoulders above any other columns in quantitative capability.
Comparison of chromatographic performance for ionic compounds with UHPLC column
| Basic compounds | |
|---|---|
| Column | 50 X 2.0 mmI.D. or 50 X 2.1 mmI.D. |
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35) |
| Flow rate | 0.2 mL/min |
| Temperature | 40℃ |
| Detection | UV at 235 nm |
| Sample | 1. Chlorpheniramine 2. Dextromethorphan 3. Propyl paraben (I.S.) |
| Coordination compound | |
|---|---|
| Column | 50 X 2.0 mmI.D. or 50 X 2.1 mmI.D. |
| Eluent | acetonitrile/0.1% H3PO4 (40/60) |
| Flow rate | 0.2 mL/min |
| Temperature | 40℃ |
| Detection | UV at 254 nm |
| Sample | 1. Hinokitiol 2. Methyl benzoate (I.S.) |
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