我應該平衡HPLC管柱多久時間呢？
在執行梯度條件後進行下一次進樣前之HPLC層析管柱平衡時，如果（重新）平衡時間太短，則會出現實驗重複性不佳的風險。如果時間太長，則會浪費時間和溶劑。
在本HPLC技術指引中，您將學習如何通過計算管柱體積來估算最佳平衡體積。此外，還使用了一個實際案例來顯示不同平衡體積對分析結果的影響。有關管柱平衡的詳細說明，您可以在此處下載HPLC技術指引。
Expert Tip: 管柱再生Column regeneration
您的(U)HPLC層析管柱之分離效果是否開始變差？
這份文件我們提供了合適的管柱清洗程序，有助於恢復層析管柱之性能。此外，該程序還可防止污染物在固定相上累積。但是，需考量一些情況，例如：清潔溶劑的類型和有效再生所需的體積。有關層析管柱再生的更多詳細信息，請在此處下載說明文件。
Improving the retention behaviour of hydrophilic compounds on polar stationary phases
Do you analyse hydrophilic compounds? Do you want to improve the retention on polar stationary phases such as PFP or Diol?
In this technical note, two different options for the improved analysis of hydrophilic compounds on YMC-Triart PFP and YMC-Triart Diol-HILIC are shown. The pH value and the amount of organic solvent are examined to learn more about the retention behaviour of basic, acidic and zwitterionic compounds using these phases. Due to their different selectivity, pentafluorophenyl (PFP) or Diol modifications are an ideal choice for the analysis of hydrophilic compounds which often remain unretained or unresolved on conventional C18 phases.
You can download the full method details here.
To get further information about the YMC-Triart series of columns.
Fast UHPLC Analysis of SARS-CoV-2 drug Remdesivir and its active metabolite GS-441524
As the COVID-19 pandemic continues, the worldwide search for an effective treatment is still ongoing. Remdesivir and its active metabolite GS-441524 are both antiviral drugs that have been examined and partially approved for the treatment of COVID-19 due to their capability of inhibiting virus replication.
In this application note, the separation of GS-441524 and Remdesivir in only 3 minutes is shown. A YMC-Triart C18 UHPLC column provides fast and reliable results making it an ideal choice for QC purposes.
You can download the application note with the full method details here.
Time-Saving of Esomeprazole Magnesium Analysis -International Harmonization of Pharmacopoeia-
The full article can be downloaded as a pdf.
USP class 管柱分類
| USP Class | Functional group | USP Description | YMC Product |
| L01 | C18 | Octadecyl silane <ODS or C18> chemically bonded to porous silica or ceramic particles – 1.5 to 10 μm in diameter |
|
| L03 | SIL | Porous silica particles – 1.5 to 10 μm in diameter | |
| L07 | C8 | Octyl silane <C8> chemically bonded to porous silica particles – 1.5 to 10 μm in diameter | |
| L08 | NH2 | An essentially monomolecular layer of aminopropylsilane <NH2> chemically bonded to totally porous silica gel support – 1.5 to 10 μm in diameter | |
| L10 | CN | Nitrile groups <CN> chemically bonded to porous silica particles – 1.5 to 10 μm in diameter | |
| L11 | Ph | Phenyl groups chemically bonded to porous silica particles – 1.5 to 10 μm in diameter | |
| L13 | C1 | Trimethylsilane <C1> chemically bonded to porous silica particles – 1.5 to 10 μm in diameter | |
| L20 | Diol | Dihydroxypropane groups chemically bonded to porous silica particles – 1.5 to 10 μm in diameter | |
| L26 | C4 | Butyl silane <C4> chemically bonded to porous silica particles – 1.5 to 10 μm in diameter | |
| L27 | SIL | Porous silica particles – 30 to 50 μm in diameter | |
| L33 | Diol | Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability | |
| L40 | Cellulose tris-3,5- dimethylphenylcarbamate |
Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 3 to 20 µm in diameter | |
| L43 | PFP | Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 µm in diameter | |
| L51 | Amylose tris-3,5- dimethylphenylcarbamate |
Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 3 to 20 µm in diameter | |
| L59 | Diol | Packing for the size exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. It is spherical (1.5 to 10 μm), silica or hybrid packing with a hydrophilic coating | |
| L62 | C30 | C30 silane bonded phase on a fully porous spherical silica, 3 to 15 µm in diameter | |
| L99 | Amylose tris-3,5- dimethylphenylcarbamate |
Amylose tris-(3,5-dimethylphenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter | |
| L111 | Polyamine | Polyamine chemically bonded to porous spherical silica particles, 5 μm in diameter | |
| L119 | Cellulose tris-3,5- dichlorophenylcarbamate |
Cellulose tris-(3,5-dichlorophenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter. |
YMC玻璃管柱選擇指引
YMC玻璃管柱可針對客戶實際應用進行最佳化選擇，對於藥物與蛋白質純化日益複雜且高純度的需求，除有多種內徑、可調式長度選擇之外，亦可針對您所需之耐壓、溫控、耐溶劑等實驗需求提供相對應的產品。並且可供應從實驗室一路放大到生產端之完整的產品線供應。以下為簡單選擇指引，詳細規格請參考各產品連結或來電與我們諮詢。
ECO Columns玻璃管柱(適用LPLC、蛋白質樣品)
-低~中壓應用
-管柱內徑: 10, 15, 25, 50, 70, 80 mm
-管柱長度: 120, 200, 450, 750, 1000 mm(管柱長度可自由調整)
-耐壓: 5 ~ 30 bar(依內徑大小耐壓規格略有差異)
–溫控夾套(選配): Heating/cooling jacket
-操作快速: Easy screw-lock 設計操作便利
ECOPLUS Columns(適用MPLC)
-中~高壓應用
-管柱內徑: 5, 10, 15, 25, 35, 50 mm
-管柱長度: 125, 250, 500 mm(管柱長度可自由調整)
-耐壓: 15 – 80 bar(依內徑大小耐壓規格略有差異)
-再現性佳
-操作快速: Quick-Lock 設計操作便利
YMC HT Columns(高溫應用)
-中壓應用
-管柱內徑: 10, 15, 25, 50 mm
-管柱長度: 120, 200, 300, 450, 750, 1000 mm(管柱長度可自由調整)
-操作溫度: 4 – 70 °C
-耐壓: 30 – 10 bar(依內徑大小耐壓規格略有差異)
-操作快速: Easy screw-lock 設計操作便利
YMC Pilot
-應用廣泛: 適用於BioLC或逆相(RP)/正相(NP)等應用
-內徑: 100, 140, 200, 300 mm
-可訂製不繡鋼材質
-可選配溫控夾套(Heating/Cooling jacket)
-操作簡單、密封(Seal)技術佳
(更大內徑需求請來信詢問)
YMC PilotPLUS
-Bio液相層析之生物相容性
-內徑: 70, 100, 140, 200 mm
-特殊排液設計使填充管柱簡單且無氣泡產生
-可拆卸式玻璃管柱設計可快速且乾淨的卸除玻璃管柱
如何選擇C18層析管柱-Guides for selecting C18 packing materials
| 小分子化合物
Low molecular weight compounds |
一般有機化合物
General organic compounds |
||
| General organic compounds | YMC-Triart C18 Meteoric Core C18 |
||
| Chemical structures differ slightly among solutes | YMC-Triart C18 ExRS | ||
| Hydrophobicity differs greatly among solutes | YMC-Triart Bio C18 J’sphere ODS-L80 |
||
| Difficult to separate using standard ODS | YMC-Triart C18 ExRS Hydrosphere C18 YMC-Pack Pro C18 RS YMC-Pack ODS-AL J’sphere ODS-H80 J’sphere ODS-L80 YMC-Pack PolymerC18 |
||
| Basic compounds | YMC-Triart C18 YMC-Triart C18 ExRS |
||
| Coordination compounds | YMC-Triart C18 YMC-Triart C18 ExRS |
||
| Sugars and glycosides | YMC-Triart C18 Hydrosphere C18 |
||
| High molecular weight compounds
大分子化合物 |
High molecular weight peptides and oligonucleic acids | YMC-Triart C18 YMC-Triart Bio C18 Meteoric Core C18 BIO |
|
| Alkaline mobile phase
鹼性移動相條件 |
YMC-Triart C18 YMC-Triart C18 ExRS YMC-Triart Bio C18 YMC-Pack PolymerC18 |
||
HPLC層析分離管柱應用方法集
為加速HPLC使用者更容易找到合適的分析管柱，YMC提供以下分析方法資料，請依照您樣品特性選擇適合的參考方法。
因資料庫龐大無法即時更新，如以下未列出您的樣品條件，請來電(02-88663356)或來信(info@ymctaiwan.com)索取。
Nucleic acid 核酸
Antibody 抗體
Amino acids / Peptdie / Protein 胺基酸/胜肽/蛋白質
Chiral 手性/掌性
SFC 超臨界
純化管柱類型
使用液相層析系統進行樣品純化時，該如何挑選適合的管柱呢?
一般市面上我們常聽到Open Column, Flash Column, Glass Column, Preparative Column(半)製備級管柱, DAC…等，
可依據下表說明有一個選擇方向，然不同廠牌間規格略有差異，請與您的系統供應商或與我們聯繫討論。
|
Open Column |
Flash Column | Glass Column | Preparative Column |
DAC |
|
| 壓力 |
常壓 |
低壓 | 低壓~中壓 | 低~中~高壓 | |
| 規模 |
實驗室~工廠生產 |
實驗室 | 實驗室~工廠生產 | 實驗室 |
試量產~工廠生產 |
| 搭配LC系統 |
不需要 |
Flash系統 | FPLC~MPLC | 製備級HPLC | |
| 常用膠體粒徑 |
不規則型 |
不規則型 | 聚合物、球型10μm ~>50μm | 聚合物、 | |
| 樣品純度* |
低 |
低 | 低~中 | 高 |
中~高 |
| 純化時間 |
慢 |
快 |
|||
| 常見應用領域 |
合成樣品、天然物 |
合成樣品 | 合成樣品、天然物 | 不純物、
難分離樣品 |
藥物生產 |
*樣品純度需視樣品複雜度略有差異，一般來說選擇膠體粒徑越小得到純度越高
小量純化之測試管柱開放申請中！
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